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Macrophages antibody | BA4D5

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Anti Pig Macrophages Antibody, clone BA4D5 thumbnail image 1 Anti Pig Macrophages Antibody, clone BA4D5 thumbnail image 2
Anti Pig Macrophages Antibody, clone BA4D5 gallery image 1
Staining of pig peripheral blood monocytes with Mouse anti Pig Macrophages (MCA2317GA) followed by Goat anti Mouse IgG:FITC (STAR70)
Anti Pig Macrophages Antibody, clone BA4D5 gallery image 2
Published customer image:
Mouse anti Porcine macrophages antibody, clone BA4D5 (MCA2317GA) used for the demonstration of macrophages in the subretinal space of pigs by immunofluorescence.
Image caption:
Immunolabeling of iPSC-RPE bolus injections in the subretinal space where the GFP-iPSC panels indicate transplanted cells (labeled in green).
The merged panels demonstrate presence of accompanying GFP-negative, non-iPSC-derived cells that are positive for macrophage (top panels), CD45 markers (middle panels), and IgG (lowest panels). Corresponding bright field panels are on the far right column. Scale bar = 100 μm.

From: Sohn EH, Jiao C, Kaalberg E, Cranston C, Mullins RF, Stone EM, Tucker BA.
Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study.
Sci Rep. 2015 Jul 3;5:11791.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Pig Macrophages:FITC

Mouse anti Pig Macrophages

Product Type
Monoclonal Antibody
Clone
BA4D5
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2317F
Datasheet
F*
SDS
0.1 mg loader

MCA2317GA
Datasheet
C F* IF IP WB*
SDS
0.1 mg loader

Mouse anti Pig Macrophages antibody, clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.

Some expression has also been observed on peripheral blood lymphocytes.

The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.

It is believed that clone BA4D5 may be specific for porcine CD68 (Poulsen et al. 2016) although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Macrophages antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
Flow Cytometry 1 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 2
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  2. 2 BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader

Product Specific References

References for Macrophages antibody

  1. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
    Eur J Cell Biol. 87: 91-9.
  2. Fujita M et al. (2013) Technique of endoscopic biopsy of islet allografts transplanted into the gastric submucosal space in pigs.
    Cell Transplant. 22 (12): 2335-44.
  3. Muscari C et al. (2010) Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage.
    Biol Proced Online. 12 (1): 9023.
  4. Liu, G. et al. (2015) Influenza A Virus Panhandle Structure is Directly Involved in RIG-I Activation and IFN Induction.
    J Virol. pii: JVI.00232-15.
  5. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
    Dev Comp Immunol. 33 (3): 284-98.
  6. Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.
  7. Poulsen, C.B. et al. (2016) Treatment with a human recombinant monoclonal IgG antibody against oxidized LDL in atherosclerosis-prone pigs reduces cathepsin S in coronary lesions.
    Int J Cardiol. 215: 506-515.
  8. Sohn, E.H. et al. (2015) Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study.
    Sci Rep. 5: 11791.
  9. Wang, L. et al. (2017) Porcine alveolar macrophage polarization is involved in inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) replication.
    J Vet Med Sci. Sep 17 [Epub ahead of print].
  10. Porras, A.M. et al. (2018) Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease.
    Proc Natl Acad Sci U S A. 115 (3): E363-E371.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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