Macrophages antibody | BA4D5
Mouse anti Porcine macrophages antibody, clone BA4D5 (MCA2317GA) used for the demonstration of macrophages in the subretinal space of pigs by immunofluorescence.
Image caption:
Immunolabeling of iPSC-RPE bolus injections in the subretinal space where the GFP-iPSC panels indicate transplanted cells (labeled in green).
The merged panels demonstrate presence of accompanying GFP-negative, non-iPSC-derived cells that are positive for macrophage (top panels), CD45 markers (middle panels), and IgG (lowest panels). Corresponding bright field panels are on the far right column. Scale bar = 100 μm.
From: Sohn EH, Jiao C, Kaalberg E, Cranston C, Mullins RF, Stone EM, Tucker BA.
Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study.
Sci Rep. 2015 Jul 3;5:11791.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig Macrophages antibody, clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.
Some expression has also been observed on peripheral blood lymphocytes.
The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.
It is believed that clone BA4D5 may be specific for porcine CD68 (Poulsen et al. 2016) although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
Some expression has also been observed on peripheral blood lymphocytes.
The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.
It is believed that clone BA4D5 may be specific for porcine CD68 (Poulsen et al. 2016) although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Macrophages antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | ||
| Flow Cytometry 1 | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting 2 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- 2 BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
|
|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
Product Specific References
References for Macrophages antibody
-
Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
Eur J Cell Biol. 87: 91-9. -
Fujita M et al. (2013) Technique of endoscopic biopsy of islet allografts transplanted into the gastric submucosal space in pigs.
Cell Transplant. 22 (12): 2335-44. -
Muscari C et al. (2010) Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage.
Biol Proced Online. 12 (1): 9023. -
Liu, G. et al. (2015) Influenza A Virus Panhandle Structure is Directly Involved in RIG-I Activation and IFN Induction.
J Virol. pii: JVI.00232-15. -
Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
Dev Comp Immunol. 33 (3): 284-98. -
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Poulsen, C.B. et al. (2016) Treatment with a human recombinant monoclonal IgG antibody against oxidized LDL in atherosclerosis-prone pigs reduces cathepsin S in coronary lesions.
Int J Cardiol. 215: 506-515. -
Sohn, E.H. et al. (2015) Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study.
Sci Rep. 5: 11791. -
Wang, L. et al. (2017) Porcine alveolar macrophage polarization is involved in inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) replication.
J Vet Med Sci. Sep 17 [Epub ahead of print]. -
Porras, A.M. et al. (2018) Creation of disease-inspired biomaterial environments to mimic pathological events in early calcific aortic valve disease.
Proc Natl Acad Sci U S A. 115 (3): E363-E371.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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