Lambda Light Chain antibody | K139 3E1
Mouse anti Pig Ig Lambda Light Chain
- Product Type
- Monoclonal Antibody
- Clone
- K139 3E1
- Isotype
- IgG2a
- Specificity
- Lambda Light Chain
Antibody Quality and Performance Guarantee
| Mouse anti Pig Ig λ light chains antibody, clone K139 3E1, recognizes the lambda light chains of porcine immunoglobulins. K139 3E1 recognizes a subpopulation of cells expressing lambda light chains (Sinkora et al.). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunized mice were fused with cells of the P3 - X63 - Ag 8.653 mouse myeloma line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5,000 | 1/100,000 |
| Flow Cytometry | |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for Lambda Light Chain antibody
-
Andersen, J.K. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
Immunology 98: 612-21. -
Sinkora, J. et al. (2001) Characterisation of monoclonal antibodies recognising kappa and lambda chains in pigs by flow cytometry.
Vet Immunol Immunopathol. 80: 79 - 91 -
Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
Dev Comp Immunol. 65: 31-40.
- UniProt
- P01846
- GO Terms
- GO:0003823 antigen binding
MCA633GA
1605
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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