Lambda Light Chain antibody | K139 3E1

Mouse anti Pig Ig Lambda Light Chain

Product Type
Monoclonal Antibody
Clone
K139 3E1
Isotype
IgG2a
Specificity
Lambda Light Chain

Product Code Applications Pack Size List Price Your Price Qty
MCA633GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F P 0.1 mg loader
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loader
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Mouse anti Pig Ig λ light chains antibody, clone K139 3E1, recognizes the lambda light chains of porcine immunoglobulins.
K139 3E1 recognizes a subpopulation of cells expressing lambda light chains (Sinkora et al.).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine IgG
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of immunised mice were fused with cells of the P3 - X63 - Ag 8.653 mouse myeloma line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/100,000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for Lambda Light Chain antibody

  1. Sinkora, J. et al. (2001) Characterisation of monoclonal antibodies recognising kappa and lambda chains in pigs by flow cytometry.
    Vet Immunol Immunopathol. 80: 79 - 91
  2. Andersen, J.K. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
    Immunology 98: 612-21.
  3. Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
    Dev Comp Immunol. 65: 31-40.

Flow Cytometry

UniProt
P01846
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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