IL-18 antibody | 7-G-8
Mouse anti Porcine interleukin-18 antibody, clone 7-G-8 recognizes natural and recombinant porcine IL-18. The antibody does not recognize porcine IL-1beta, IL-8, IL-12 or IFNγ, nor does it cross react with human or murine IL-18.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O19073
- Entrez Gene
- IL18
- GO Terms
- GO:0001525 angiogenesis
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- Regulatory
- For research purposes only
Applications of IL-18 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5ug/ml as coating antibody | ||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.
- ELISA
- This antibody may be used as a capture reagent in an sandwich ELISA pair with MCA2094B as detection antibody.
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Secondary Antibodies Available
Product Specific References
References for IL-18 antibody
-
Muneta, Y. et al. (2000) Detection of porcine interleukin-18 by sandwich ELISA and immunohistochemical staining using its monoclonal antibodies.
J Interferon Cytokine Res. 20 (3): 331-6. -
Lee, B.M. et al. (2011) Enhanced protection against infection with transmissible gastroenteritis virus in piglets by oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing swine interferon-α and interleukin-18.
Comp Immunol Microbiol Infect Dis. 34 (4): 369-80.
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