Granulocytes antibody | 6D10

Mouse anti Pig Granulocytes

Product Type
Monoclonal Antibody
Clone
6D10
Isotype
IgG2a
Specificity
Granulocytes
Region
(NEUTROPHIL LINEAGE)

Product Code Applications Pack Size List Price Your Price Qty
MCA2599GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB * 0.1 mg loader
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loader
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Mouse anti Pig Granulocytes antibody, clone 6D10 recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.

Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.

Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérez et al. 2007).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine bone marrow haematopoietic cells (BMHC).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mouse were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunoprecipitation
Western Blotting 1
  1. 1Clone 6D10 recongnizes an antigen from porcine granulocytes under non-reducing conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA2599GA detects a band of approximately 60kDa in porcine BMHC cell lysates.

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References for Granulocytes antibody

  1. Pérez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
    Dev Comp Immunol. 31 (3): 296-306.
  2. Stone, J.P. et al. (2016) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes Using Ex Vivo Lung Perfusion.
    Am J Transplant. 16 (1): 33-43.
  3. Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
    Dev Comp Immunol. 33 (3): 284-98.
  4. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  5. Nguyen, D.N. et al. (2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
    Sci Rep. 6: 36816.
  6. Andersen, A.D. et al. (2019) Synbiotics Combined with Glutamine Stimulate Brain Development and the Immune System in Preterm Pigs.
    J Nutr. 149 (1): 36-45.
  7. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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