CD9 antibody | 4H5CR4
Mouse anti Pig CD9
- Product Type
- Monoclonal Antibody
- Clone
- 4H5CR4
- Isotype
- IgG1
- Specificity
- CD9
Antibody Quality and Performance Guarantee
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F Reset| Mouse anti Pig CD9 antibody, clone 4H5CR4 recognizes pig CD9. CD9 is a member of the tetraspanin protein family and is involved in various cellular processes such as cell adhesion, proliferation, migration and signal transduction. Similar to its expression in humans, pig CD9 is found on a diverse array of cell types, such as platelets, lymphocytes, and endothelial cells. Mouse anti-Pig CD9 antibody, clone 4H5CR4, has been shown to detect CD9 on porcine cells. The use of Mouse anti-Pig CD9 in combination with antibodies to CD4, CD8α and 2E3 makes it possible to distinguish five main CD4+ T cell subsets, making it a useful marker to investigate CD4+ T cell heterogeneity in porcine models (Alvarez et al. 2022). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is sold under a license from Spanish National Research Council (CSIC).
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD9 antibody
-
Alvarez, B. et al. (2022) CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells.
Dev Comp Immunol. 133
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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