CD46 antibody | JM6C11

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Anti Pig CD46 Antibody, clone JM6C11 thumbnail image 1

Anti Pig CD46 Antibody, clone JM6C11 thumbnail image 2

Anti Pig CD46 Antibody, clone JM6C11 thumbnail image 3

Anti Pig CD46 Antibody, clone JM6C11 thumbnail image 4

Anti Pig CD46 Antibody, clone JM6C11 gallery image 1 — Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD46 (**MCA2262GA**).

Anti Pig CD46 Antibody, clone JM6C11 gallery image 2 — Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD46 (**MCA2262GA**) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).

Anti Pig CD46 Antibody, clone JM6C11 gallery image 3 — Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD46 (**MCA2262GA**) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Anti Pig CD46 Antibody, clone JM6C11 gallery image 4 — Figure A. Purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD46 (**MCA2262GA**) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD11a FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse anti Pig CD46

Product Type: Monoclonal Antibody
Clone: JM6C11
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2262GA	F			0.1 mg

Mouse anti Pig CD46 antibody, clone JM6C11 recognizes porcine CD46, also known as membrane cofactor protein (MCP). CD46 is a ~45-65 kDa single pass type 1 transmembrane glycoprotein expressed by all peripheral blood cells.

Mouse anti Pig CD46 antibody, clone JM6C11 inhibits functional MCP cofactor activity as measured by inhibition of cleavage of the α chain of C3 (Pérez De La Lastra et al. 1999).

Product Details

Target Species: Pig
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Porcine peripheral blood mononuclear cells
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch

More Information

UniProt: O02839 Related reagents
Entrez Gene: CD46 Related reagents
GO Terms: GO:0002079 inner acrosomal membrane; GO:0016021 integral to membrane; GO:0006958 complement activation, classical pathway; GO:0007338 single fertilization; GO:0045087 innate immune response
Regulatory: For research purposes only

Applications of CD46 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Functional Assays

Western Blotting

Product Specific References

References for CD46 antibody

van den Berg, C.W. et al. (1997) Purification and characterization of the pig analogue of human membrane cofactor protein (CD46/MCP).
J Immunol. 158: 1703-9.
Haverson, K. et al. (2001) Overview of the Third International Workshop on Swine Leukocyte Differentiation Antigens.
Vet Immunol Immunopathol. 80: 5-23.
Pérez De La Lastra, J.M. et al. (1999) Epitope mapping of 10 monoclonal antibodies against the pig analogue of human membrane cofactor protein (MCP).
Immunology. 96: 663-70.
Dräger, C. et al. (2015) Porcine complement regulatory protein CD46 and heparan sulfates are the major factors for classical swine fever virus attachment in vitro.
Arch Virol. 160: 739-46.