CD46 antibody | JM6C11

Product Code Applications Pack Size List Price Your Price Qty
MCA2262GA
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SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Mouse anti Pig CD46 antibody, clone JM6C11 recognizes porcine CD46, also known as membrane cofactor protein (MCP). CD46 is a ~45-65 kDa single pass type 1 transmembrane glycoprotein expressed by all peripheral blood cells.

Mouse anti Pig CD46 antibody, clone JM6C11 inhibits functional MCP cofactor activity as measured by inhibition of cleavage of the α chain of C3 (Pérez De La Lastra et al. 1999).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood mononuclear cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul

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References for CD46 antibody

  1. van den Berg, C.W. et al. (1997) Purification and characterization of the pig analogue of human membrane cofactor protein (CD46/MCP).
    J Immunol. 158: 1703-9.
  2. Haverson, K. et al. (2001) Overview of the Third International Workshop on Swine Leukocyte Differentiation Antigens.
    Vet Immunol Immunopathol. 80: 5-23.
  3. Pérez De La Lastra, J.M. et al. (1999) Epitope mapping of 10 monoclonal antibodies against the pig analogue of human membrane cofactor protein (MCP).
    Immunology. 96: 663-70.
  4. Dräger, C. et al. (2015) Porcine complement regulatory protein CD46 and heparan sulfates are the major factors for classical swine fever virus attachment in vitro.
    Arch Virol. 160: 739-46.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Functional Assays

Western Blotting

Synonyms
Membrane CO-FACTOR Protein
RRID
AB_1102608
UniProt
O02839
Entrez Gene
CD46
GO Terms
GO:0002079 inner acrosomal membrane
GO:0016021 integral to membrane
GO:0006958 complement activation, classical pathway
GO:0007338 single fertilization
GO:0045087 innate immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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