CD46 antibody | 6D8/8
Mouse anti Pig CD46
- Product Type
- Monoclonal Antibody
- Clone
- 6D8/8
- Isotype
- IgG1
- Specificity
- CD46
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD46, clone 6D8/8 recognizes the porcine CD46 cell surface antigen, a ~50-60 kDa glycoprotein also known as membrane cofactor protein (MCP). Porcine CD46 is expressed on a wide range of cells including leucocytes, platelets and erythrocytes. CD46 serves as an inhibitor of complement activation on host cells. Mouse anti Pig CD46, clone 6D8/8 induces homotypic cell aggregation, (Bullido et al. 2000). . Mouse anti pig CD46, clone 6D8/8 detects a series of closely apposed bands of approximately 50-60 kDa in pig erythrocyte lysates (Perez De La Lastra et al. 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD46 antibody
-
Perez de la Lastra, J.M. et al. (1999) Epitope mapping of 10 monoclonal antibodies against the pig analogue of human membrane cofactor protein (MCP).
Immunology 96: 663-670. -
Bullido, R. et al. (2000) Induction of aggregation in porcine lymphoid cells by antibodies to CD46.
Vet Immunol Immmunopathol. 73: 73-81. -
Dräger, C. et al. (2015) Porcine complement regulatory protein CD46 and heparan sulfates are the major factors for classical swine fever virus attachment in vitro.
Arch Virol. 160: 739-46.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- Membrane CO-FACTOR Protein
- UniProt
- O02839
- Entrez Gene
- CD46
- GO Terms
- GO:0002079 inner acrosomal membrane
- GO:0016021 integral to membrane
- GO:0006958 complement activation, classical pathway
- GO:0007338 single fertilization
- GO:0045087 innate immune response
MCA2310GA
1601
161373
167515
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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