CD33 antibody | 5D5
Mouse anti Pig CD33
- Product Type
- Monoclonal Antibody
|Mouse anti Pig CD33, clone 5D5 recognizes CD33, also known as Siglec-3.
Sialic-acid-binding immunoglobulin-like lectin (Siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses.
Clone 5D5 was used to detect Siglec-3 expression on monocytes (CD172a+ or CD16hi PBMCs), granulocytes (CD52+ and CD52-), and bone marrow. CD33 was also detected in lymph node, splenic, and alveolar macrophages, although at lower levels than on monocytes (Alvarez et al. 2015).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant protein consisting in the two extracellular Ig-like domains of porcine Siglec-3 fused to the Fc portion of human IgG1 (pSiglec- 3-Fc)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD33 antibody
Álvarez, B. et al. (2015) Molecular and functional characterization of porcine Siglec-3/CD33 and analysis of its expression in blood and tissues.
Dev Comp Immunol. 51 (2): 238-50.
Chamorro, S. et al. (2005) Phenotypic and functional heterogeneity of porcine blood monocytes and its relation with maturation.
Immunology. 114 (1): 63-71.
Bohorquez, J.A. et al. (2019) Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host.
Viruses. 11 (9)Sep 04 [Epub ahead of print].
Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
Comp Immunol Microbiol Infect Dis. 64: 31-39.
Poderoso, T. et al. (2020) Expression of Siglec-1, -3, -5 and -10 in porcine cDC1 and cDC2 subsets from blood, spleen and lymph nodes and functional capabilities of these cells.
Dev Comp Immunol. 109: 103692.
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