CD282 antibody | 1H11
Mouse anti Pig CD282
- Product Type
- Monoclonal Antibody
|Mouse anti Pig CD282, clone 1H11 recognizes CD282, also known as toll-like receptor 2 (TLR2).
Toll like receptors are a family of pattern-recognition receptors involved in the activation and regulation of inflammatory and immune responses activated by invasion of host tissues by microbial pathogens. TLR2 plays a role in a range of infections including gram-positive bacteria, mycobacteria, and yeast. It is activated by PAMPs, signals to NF-kappa-B which in turn induces cytokine secretion and the inflammatory response.
Clone 1H11 was used to characterize the expression of porcine TLR2; cells of the innate immune system - monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes were found to express porcine TLR2. Swine neutrophils express low levels of TLR2. It is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis (Alvarez et al 2008).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- NIH/3T3 mouse cells transfected with plasmid pTLR2-GFP, encoding the porcine TLR2 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD282 antibody
Alvarez, B. et al. (2008) Expression of toll-like receptor 2 (TLR2) in porcine leukocyte subsets and tissues.
Vet Res. 39 (2): 13.
Alvarez, B. et al. (2013) Antigen targeting to APC: from mice to veterinary species.
Dev Comp Immunol. 41 (2): 153-63.
Chamorro, S. et al. (2005) Phenotypic and functional heterogeneity of porcine blood monocytes and its relation with maturation.
Immunology. 114 (1): 63-71.
Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
Comp Immunol Microbiol Infect Dis. 64: 31-39.
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