CD28 antibody | 3D11
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Mouse anti Pig CD28
- Product Type
- Monoclonal Antibody
- Clone
- 3D11
- Isotype
- IgG1
- Specificity
- CD28
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD28 antibody, clone 3D11 recognizes pig CD28. CD28 is a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Analogous to humans, CD28 is expressed on naive CD4+ and CD8+ T cells and nearly all CD4+ T cells with a central memory phenotype (CD8a+ CD27+ in pigs) with the latter expressing higher levels of CD28 than naive T cells. Approximately 50% of CD4+ effector memory T cells bear CD28; at levels similar to central memory CD4+ T cells. Mouse anti Pig CD28 antibody, clone 3D11, has been shown to be able to detect CD28 on CD8+ and CD4+ αβ T-cells (Uehlein et al. 2021). Among γδ T cells, a small CD2+ subpopulation of phenotypically naive cells express CD28. In vitro, clone 3D11 can co-stimulate porcine T cells, inducing increased proliferation and cytokine secretion. |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- A20J cells expressing pig CD28
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Antibody Characterization Reference
-
Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
J Immunol. 207 (10): 2473-2488.
- UniProt
- A0A5G2R2W4
MCA6406GA
167329
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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