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CD28 antibody | 3D11

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New MCA6406GA
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SDS Safety Datasheet SDS
F 0.1 mg loader
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loader

Mouse anti Pig CD28 antibody, clone 3D11 recognizes pig CD28.

CD28 is a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Analogous to humans, CD28 is expressed on naive CD4+ and CD8+ T cells and nearly all CD4+ T cells with a central memory phenotype (CD8a+ CD27+ in pigs) with the latter expressing higher levels of CD28 than naive T cells. Approximately 50% of CD4+ effector memory T cells bear CD28; at levels similar to central memory CD4+ T cells.

Mouse anti Pig CD28 antibody, clone 3D11, has been shown to be able to detect CD28 on CD8+ and CD4+ αβ T-cells (Uehlein et al. 2021). Among γδ T cells, a small CD2+ subpopulation of phenotypically naive cells express CD28. In vitro, clone 3D11 can co-stimulate porcine T cells, inducing increased proliferation and cytokine secretion.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
A20J cells expressing pig CD28
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Antibody Characterization Reference

  1. Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
    J Immunol. 207 (10): 2473-2488.

UniProt
A0A5G2R2W4

MCA6406GA

167329

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