CD18a antibody | PNK-I
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F ResetMouse anti Pig CD18a:FITC
- Product Type
- Monoclonal Antibody
- Clone
- PNK-I
- Isotype
- IgG1
- Specificity
- CD18a
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD18a, clone PNK-I recognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin β2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990). CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules. CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chen et al. 2011). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Camel - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Porcine large granular lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD18a antibody
-
Dato, M.E. & Kim, Y.B. (1990) Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells.
J Immunol. 144 (11): 4452-62. -
Haverson, K. et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics.
Immunology 96: 66-73. -
Vanden Bergh, P.G. et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.
Vet Res. 40:1-10. -
Chen, Z.W. et al. (2011) Mechanisms underlying Actinobacillus pleuropneumoniae exotoxin ApxI induced expression of IL-1β, IL-8 and TNF-α in porcine alveolar macrophages.
Vet Res. 42:25. -
Vanden Bergh, P.G. et al. (2008) Probing of Actinobacillus pleuropneumoniae ApxIIIA toxin-dependent cytotoxicity towards mammalian peripheral blood mononucleated cells.
BMC Res Notes 1:121. -
Ebdrup, L. et al. (2008) Dynamic expression of the signal regulatory protein alpha and CD18 on porcine PBMC during acute endotoxaemia.
Scand J Immunol. 68:430-7.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39:54.
- Synonyms
- Integrin Beta 2 Chain
- RRID
- AB_2296305
- UniProt
- P53714
- Entrez Gene
- ITGB2
- GO Terms
- GO:0004872 receptor activity
- GO:0005488 binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007275 multicellular organismal development
MCA1972F
1015
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