CD172a antibody | BL1H7

Mouse anti Pig CD172a

Product Type
Monoclonal Antibody
Clone
BL1H7
Isotype
IgG1
Specificity
CD172a

Product Code Applications Pack Size List Price Your Price Qty
MCA2312GA
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SDS Safety Datasheet SDS
C F IP P * WB * 0.1 mg loader
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loader
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Mouse anti Pig CD172a, clone BL1H7 recognizes porcine CD172a, a member of the signal regulatory protein (SIRP) family (Alvarez et al. 2000).

Mouse anti Pig CD172a, clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haverson et al. 2001;Thacker et al. 2001). CD172a is expressed on monocyte derived dendritic cells (MoDCs) (Facci et al. 2010) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci; Jeong et al. 2010). Mouse anti Pig CD172a, clone BL1H7 immunoprecipitates a single band of ~90-110 kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions (Alvarez et al. 2000). Aberrant expression of CD172a has been noted on porcine leukemias (Sipos et al. 2006) with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC (Chamorro et al. 2005).

Mouse anti Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues (Domenech et al. 2003).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 2
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2Clone BL1H7 recognizes porcine CD172a under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
Histology Positive Control Tissue
Porcine spleen.
Western Blotting
MCA2312GA detects a band of approximately 90-115 kDa in alveolar macrophage lysates.

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References for CD172a antibody

  1. Alvarez, B. et al. (2000) A porcine cell surface receptor identified by monoclonal antibodies to SWC3 is a member of the signal regulatory protein family and associates with protein-tyrosine phosphatase SHP-1.
    Tissue Antigens. 55 (4): 342-51.
  2. Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
    Vet Immunol Immunopathol. 94 (1-2): 77-81.
  3. Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
    Vet Immunol Immunopathol. 89 (1-2): 91-8.
  4. Fraile, L. et al. (2012) Immunomodulatory properties of beta-sitosterol in pig immune responses.
    Int Immunopharmacol. 13 (3): 316-21.
  5. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Clin Vaccine Immunol. 17 (4): 503-12.
  6. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
    Vet Res. 42: 9.
  7. Moreno, S. et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
    Vet Res. 41: 76.
  8. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
    Immunology. 129: 396-405.
  9. View The Latest Product References
  10. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
    Vet Immunol Immunopathol. 104: 131-44.
  11. Argilaguet, J.M. et al. (2012) DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies.
    PLoS One. 7 (9): e40942.
  12. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  13. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
    Cell Biol Int. 36 (12): 1233-8.
  14. Robinson, S.R. et al. (2015) Broadly neutralizing antibodies against the rapidly evolving porcine reproductive and respiratory syndrome virus.
    Virus Res. 203: 56-65.
  15. Li, J. & Murtaugh, M.P. (2015) Functional analysis of porcine reproductive and respiratory syndrome virus N-glycans in infection of permissive cells.
    Virology. 477: 82-8.
  16. Prims. S. et al. (2016) Intestinal immune cell quantification and gram type classification of the adherent microbiota in conventionally and artificially reared, normal and low birth weight piglets.
    J Livestock Sci 185: 1-7.
  17. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  18. Valekova I et al. (2016) Revelation of the IFNα, IL-10, IL-8 and IL-1β as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model.
    J Neuroimmunol. 293: 71-81.
  19. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  20. Thirion-Delalande, C. et al. (2017) Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
    PLoS One. 12 (9): e0183398.
  21. Auray, G. et al. (2013) Porcine neonatal blood dendritic cells, but not monocytes, are more responsive to TLRs stimulation than their adult counterparts.
    PLoS One. 8 (5): e59629.
  22. Hu, W. et al. (2021) shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus.
    Sci Rep. 11 (1): 16377.
  23. Radlowski, E.C. et al. (2021) Combination-Feeding Causes Differences in Aspects of Systemic and Mucosal Immune Cell Phenotypes and Functions Compared to Exclusive Sow-Rearing or Formula-Feeding in Piglets.
    Nutrients. 13(4):1097.
  24. Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
    Vaccines. 10 (5): 684.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Synonyms
SWC3
UniProt
Q5K4Q3
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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