CD11c antibody | 3A8
Mouse anti Pig CD11c
- Product Type
- Monoclonal Antibody
- Clone
- 3A8
- Isotype
- IgG1
- Specificity
- CD11c
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD11c, clone 3A8 recognizes CD11c also known as integrin alpha-X. Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC). Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells (Deloizy et al. 2016). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Pig alveolar macrophages
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Antibody Characterization Reference
-
Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
Dev Comp Immunol. 65: 31-40.
References for CD11c antibody
-
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16) :2103.
- UniProt
- K9IVW2
MCA6150GA
159406
167902
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD11c specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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