CD1 antibody | 76-7-4

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Mouse anti Pig CD1:FITC

Mouse anti Pig CD1

Mouse anti Pig CD1:RPE

Product Type
Monoclonal Antibody
Clone
76-7-4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6098F F 0.1 mg
MCA6098GA F 0.1 mg
MCA6098PE F 0.1 mg
Mouse anti Pig CD1 antibody, clone 76-7-4 recognizes a porcine homologue of the human CD1 cell surface receptor. CD1 is a member of the immunoglobulin superfamily. While it is structurally similar to the MHC class I receptor CD1 differs functionally by presenting lipid antigens to T cells (Germain & Margulies, 1993).

To date, five classes of the CD1 genes have been identified across different mammalian species, namely CD1A, CD1B, CD1C, CD1D, and CD1E (Brigl & Brenner, 2004). In swine various tissues express CD1C even though its gene has been reported to carry a loss-of-function mutation (Eguchi-Ogawa et al. 2007). CD1 is predominantly found on dendritic cells, macrophages, monocytes, and thymocytes (Chun et al. 1999).

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
<10% Sucrose
Immunogen
Fresh dd miniature swine thymocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch
Shelf Life
Please see the label for the expiry

More Information

UniProt
Q9XS72 Related reagents
Entrez Gene
CD1.1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation
Regulatory
For research purposes only

Applications of CD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig CD45RA:RPE MCA1751PE 100 Tests F
Mouse anti Pig CD14 MCA1218GA 0.1 mg C F IF
Mouse anti Pig CD45RA MCA1751GA 0.1 mg C F P
Mouse anti Pig CD14:FITC MCA1218F 0.1 mg F
Mouse anti Pig CD45RA:FITC MCA1751F 0.1 mg F

Product Specific References

Source Reference

  1. Pescovitz, M.D. et al. (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL.
    J Immunol. 133 (1): 368-75.

References for CD1 antibody

  1. Pauly, T. et al. (1998) Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.
    J Gen Virol. 79 ( Pt 1): 31-40.