CD1 antibody | 76-7-4
Mouse anti Pig CD1 antibody, clone 76-7-4 recognizes a porcine homologue of the human CD1 cell surface receptor. CD1 is a member of the immunoglobulin superfamily. While it is structurally similar to the MHC class I receptor CD1 differs functionally by presenting lipid antigens to T cells (Germain & Margulies, 1993).
To date, five classes of the CD1 genes have been identified across different mammalian species, namely CD1A, CD1B, CD1C, CD1D, and CD1E (Brigl & Brenner, 2004). In swine various tissues express CD1C even though its gene has been reported to carry a loss-of-function mutation (Eguchi-Ogawa et al. 2007). CD1 is predominantly found on dendritic cells, macrophages, monocytes, and thymocytes (Chun et al. 1999).
To date, five classes of the CD1 genes have been identified across different mammalian species, namely CD1A, CD1B, CD1C, CD1D, and CD1E (Brigl & Brenner, 2004). In swine various tissues express CD1C even though its gene has been reported to carry a loss-of-function mutation (Eguchi-Ogawa et al. 2007). CD1 is predominantly found on dendritic cells, macrophages, monocytes, and thymocytes (Chun et al. 1999).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
<10% Sucrose - Immunogen
- Fresh dd miniature swine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
- Shelf Life
- Please see the label for the expiry
More Information
- UniProt
- Q9XS72 Related reagents
- Entrez Gene
- CD1.1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
- Regulatory
- For research purposes only
Applications of CD1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD45RA:RPE | MCA1751PE | 100 Tests | F | ||
| Mouse anti Pig CD14 | MCA1218GA | 0.1 mg | C F IF | ||
| Mouse anti Pig CD45RA | MCA1751GA | 0.1 mg | C F P | ||
| Mouse anti Pig CD14:FITC | MCA1218F | 0.1 mg | F | ||
| Mouse anti Pig CD45RA:FITC | MCA1751F | 0.1 mg | F |
Product Specific References
Source Reference
-
Pescovitz, M.D. et al. (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL.
J Immunol. 133 (1): 368-75.
References for CD1 antibody
-
Pauly, T. et al. (1998) Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.
J Gen Virol. 79 ( Pt 1): 31-40.
