CD1 antibody | 76-7-4
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F ResetMouse anti Pig CD1
- Product Type
- Monoclonal Antibody
- Clone
- 76-7-4
- Isotype
- IgG2a
- Specificity
- CD1
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| Mouse anti Pig CD1 antibody, clone 76-7-4 recognizes a porcine homologue of the human CD1 cell surface receptor. CD1 is a member of the immunoglobulin superfamily. While it is structurally similar to the MHC class I receptor CD1 differs functionally by presenting lipid antigens to T cells (Germain & Margulies, 1993). To date, five classes of the CD1 genes have been identified across different mammalian species, namely CD1A, CD1B, CD1C, CD1D, and CD1E (Brigl & Brenner, 2004). In swine various tissues express CD1C even though its gene has been reported to carry a loss-of-function mutation (Eguchi-Ogawa et al. 2007). CD1 is predominantly found on dendritic cells, macrophages, monocytes, and thymocytes (Chun et al. 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Fresh dd miniature swine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Source Reference
-
Pescovitz, M.D. et al. (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL.
J Immunol. 133 (1): 368-75.
References for CD1 antibody
-
Pauly, T. et al. (1998) Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.
J Gen Virol. 79 ( Pt 1): 31-40.
- UniProt
- Q9XS72
- Entrez Gene
- CD1.1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
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