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CD1 antibody | 76-7-4

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Anti Pig CD1 Antibody, clone 76-7-4 thumbnail image 1 Anti Pig CD1 Antibody, clone 76-7-4 thumbnail image 2
Anti Pig CD1 Antibody, clone 76-7-4 gallery image 1
Figure A. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and RPE conjugated Mouse anti Porcine CD1 (MCA6098PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Pig CD1 Antibody, clone 76-7-4 gallery image 2
Figure A. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and RPE conjugated Mouse anti Porcine CD1 (MCA6098PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Pig CD1:FITC

Mouse anti Pig CD1

Mouse anti Pig CD1:RPE

Product Type
Monoclonal Antibody
Clone
76-7-4
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6098F
Datasheet
F
SDS
0.1 mg loader

MCA6098GA
Datasheet
F
SDS
0.1 mg loader

MCA6098PE
Datasheet
F
SDS
0.1 mg loader

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
<10% Sucrose
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
Q9XS72
Entrez Gene
CD1.1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation
Regulatory
For research purposes only

Applications of CD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Pig CD45RA:RPE MCA1751PE F 100 Tests loader
Mouse anti Pig CD14 MCA1218GA C F IF 0.1 mg loader
Mouse anti Pig CD45RA MCA1751GA C F P 0.1 mg loader
Mouse anti Pig CD14:FITC MCA1218F F 0.1 mg loader
Mouse anti Pig CD45RA:FITC MCA1751F F 0.1 mg loader

Product Specific References

Source Reference

  1. Pescovitz, M.D. et al. (1984) Preparation and characterization of monoclonal antibodies reactive with porcine PBL.
    J Immunol. 133 (1): 368-75.

References for CD1 antibody

  1. Pauly, T. et al. (1998) Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.
    J Gen Virol. 79 ( Pt 1): 31-40.

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