Phosphotyrosine antibody | PY20

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Mouse anti Phosphotyrosine:Biotin

Mouse anti Phosphotyrosine:HRP

Mouse anti Phosphotyrosine

Product Type
Monoclonal Antibody
Clone
PY20
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2472B E IF WB* 0.1 mg
MCA2472P E 0.5 ml
MCA2472 E IF WB* 0.5 mg
Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.

The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.

The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.

The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

Product Details

Target Species
Chemical
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Preparation
Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.01% Thiomersal
50% Glycerol
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Phosphotyrosine antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Western Blotting 1
ELISA 1/1000 1/4000
ELISA 100ng/ml
Immunofluorescence
Western Blotting 1 2ug/ml
  1. 1For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
  1. 1For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Phosphotyrosine antibody

  1. Ruff-Jamison, S. et al. (1991) Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features.
    J Biol Chem. 266 (10): 6607-13.
  2. Takagi, S. et al. (1991) Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells.
    J Exp Med. 174 (2): 381-8.
  3. Glenney, J.R. Jr. et al. (1988) Monoclonal antibodies to phosphotyrosine.
    J Immunol Methods. 109 (2): 277-85.
  4. Vendel,A.C. et al. (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
    J Immunol. 182: 1509-17.