Phosphotyrosine antibody | PY20

Mouse anti Phosphotyrosine:Biotin
Mouse anti Phosphotyrosine:HRP
Mouse anti Phosphotyrosine
- Product Type
- Monoclonal Antibody
- Clone
- PY20
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2472B | E IF WB | 0.1 mg |
![]() |
|
MCA2472P | E | 0.5 ml |
![]() |
|
MCA2472 | E IF WB | 0.5 mg |
![]() |
The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.
The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.
The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Product Details
- Target Species
- Chemical
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography.
- Preparation
- Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.01% Thiomersal
50% Glycerol - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Phosphotyrosine antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunofluorescence | |||
Western Blotting | |||
ELISA | 1/1000 | 1/4000 | |
ELISA | 100ng/ml | ||
Immunofluorescence | |||
Western Blotting | 2ug/ml |
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Phosphotyrosine antibody
-
Ruff-Jamison, S. et al. (1991) Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features.
J Biol Chem. 266 (10): 6607-13. -
Takagi, S. et al. (1991) Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells.
J Exp Med. 174 (2): 381-8. -
Glenney, J.R. Jr. et al. (1988) Monoclonal antibodies to phosphotyrosine.
J Immunol Methods. 109 (2): 277-85. -
Vendel,A.C. et al. (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
J Immunol. 182: 1509-17.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra