Pan Ubiquitin antibody | P4D1
|Mouse anti pan ubiquitin antibody, clone P4D1 recognizes both mono- and polyubiquitin chains. The antibody has also been reported to recognize free polyubiquitin chains and free ubiquitin.
Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. The protein is covalently linked to selected lysine residues in a post-translational modification process known as ubiquitylation or ubiquitination. This chemical reaction is mediated by three different protein families; ubiquitin-activating enzymes (also known as E1s), ubiquitin-conjugating enzymes (also known as E2s) and ubiquitin ligases (also known as E3s) (Hershko and Ciechanover 1998).
The impact of ubiquitination depends on whether a single ubiquitin moiety (monoubiquitination) or an ubiquitin chain (polyubiquitination) has been attached to a protein. Monoubiquitination tends to trigger cellular processes related to endocytosis and membrane trafficking (Haglund et al. 2003) while the impact of polyubiquitination varies depending on how the ubiquitin residues in the chain have been linked. Attachment of Lysine-48 ubiquitin chains results in degradation by the 20S proteasome while addition of Lysine-63 ubiquitin chains mediates DNA damage and NFkappaB signaling (Chen 2005 and Mocciaro and Rape 2012). Lysine-6, Lysine-11, Lysine-27, Lysine-29 and Lysine-33 chains have also been reported (Komander 2009 and Ye and Rape 2011).
When comparing staining of mouse anti pan ubiquitin antibody (clone P4D1) against staining with mouse anti polyubiquitin antibody (clone FK1) one can determine, if a protein target is mono- or polyubiquitinated. In contrast to mouse anti mono- and polyubiquitin antibody (FK2), clone P4D1 also recognizes free ubiquitin.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Denatured bovine ubiquitin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Western Blotting
- Mouse anti pan ubiquitin recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin by Western Blot. Use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
References for Pan Ubiquitin antibody
Fujimuro, M. et al (1994) Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
FEBS Lett. 349 (2):173-80.
Wang, H. et al. (2008) Analysis of nondegradative protein ubiquitylation with a monoclonal antibody specific for lysine-63-linked polyubiquitin.
Proc Natl Acad Sci U S A. 105 (51): 20197-202.
Kahana, A and Gottschling, DE. (1999) DOT4 links silencing and cell growth in Saccharomyces cerevisiae.
Mol Cell Biol. 19 (10): 6608-20.
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