Huntingtin antibody | HDB4E10

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Mouse anti Huntingtin

Product Type
Monoclonal Antibody
Clone
HDB4E10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2050 C* IP WB 0.1 mg
Mouse anti Huntingtin antibody, clone HDB4E10 reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.

Mouse anti Huntingtin antibody, clone HDB4E10 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. The combined use of Mouse anti Huntingtin antibody, clones HDB4E10 (MCA2050) and HDC8A4 (MCA2051) demonstrate that huntingtin is enriched in neuronal cells in the brain (Jones 1999).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P42858 Related reagents
Entrez Gene
HTT Related reagents
GO Terms
GO:0000132 establishment of mitotic spindle orientation
GO:0002039 p53 binding
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005624 membrane fraction
GO:0005634 nucleus
GO:0005770 late endosome
GO:0005776 autophagic vacuole
GO:0005794 Golgi apparatus
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER
GO:0007030 Golgi organization
GO:0030424 axon
GO:0030425 dendrite
GO:0030659 cytoplasmic vesicle membrane
GO:0034452 dynactin binding
GO:0043234 protein complex
GO:0045505 dynein intermediate chain binding
GO:0047496 vesicle transport along microtubule
Regulatory
For research purposes only

Applications of Huntingtin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1
Immunoprecipitation
Western Blotting
  1. 1 Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunoprecipitation

Product Specific References

References for Huntingtin antibody

  1. Wilkinson, F. L. et al. (1999) Localization of rabbit huntingtin using a new panel of monoclonal antibodies.
    Molecular Brain Research. 69: 10-20.
  2. Yao Y et al. (2015) A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.
    Elife. 4: 4.
  3. Shirasaki, D.I. et al. (2012) Network organization of the huntingtin proteomic interactome in mammalian brain.
    Neuron. 75 (1): 41-57.
  4. Jones, A.L. (1999) The localization and interactions of huntingtin.
    Philos Trans R Soc Lond B Biol Sci. 354 (1386): 1021-7.
  5. Tao, T. & Tartakoff, A.M. (2001) Nuclear relocation of normal huntingtin.
    Traffic. 2 (6): 385-94.
  6. Massai, L. et al. (2013) Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells.
    BMC Biochem. 14: 34.
  7. Ni, C.L. et al. (2016) Polyglutamine Tract Expansion Increases S-Nitrosylation of Huntingtin and Ataxin-1.
    PLoS One. 11 (9): e0163359.