TRF1 antibody | BED5

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Mouse anti Human TRF1

Product Type
Monoclonal Antibody
Clone
BED5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2643GA C E P* 0.1 mg
Mouse anti human TRF1 antibody, clone BED5 recognizes TTAGGG repeat binding factor 1 (TRF1) a ubiquitously expressed 60kDa protein. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage.

TRF1 is thought to play a role in leukaemia being expressed at significantly higher levels in patients with acute lymphoblastic leukaemia than those with myeloid leukaemia (Ohyashiki et al. 2001).

Mouse anti human TRF1 antibody, clone BED5 has been reported to work in Western blotting applications.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant TRF1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P54274 Related reagents
Entrez Gene
TERF1 Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000781 chromosome, telomeric region
GO:0000783 nuclear telomere cap complex
GO:0001309 age-dependent telomere shortening
GO:0003691 double-stranded telomeric DNA binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005819 spindle
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0042162 telomeric DNA binding
GO:0007004 telomere maintenance via telomerase
GO:0007067 mitosis
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0008017 microtubule binding
GO:0008301 DNA bending activity
GO:0042802 identical protein binding
GO:0008656 caspase activator activity
GO:0010521 telomerase inhibitor activity
GO:0010834 telomere maintenance via telomere shortening
GO:0031116 positive regulation of microtubule polymerization
GO:0032211 negative regulation of telomere maintenance via telomerase
GO:0032214 negative regulation of telomere maintenance via semi-conservative replication
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0045449 regulation of transcription
GO:0045840 positive regulation of mitosis
GO:0045931 positive regulation of mitotic cell cycle
GO:0046982 protein heterodimerization activity
GO:0051301 cell division
GO:0051974 negative regulation of telomerase activity
Regulatory
For research purposes only

Applications of TRF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/200 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for TRF1 antibody

  1. Galati, A. et al. (2006) The human telomeric protein TRF1 specifically recognizes nucleosomal binding sites and alters nucleosome structure.
    J Mol Biol. 360 (2): 377-85.
  2. de Lange, T. (2005) Shelterin: the protein complex that shapes and safeguards human telomeres.
    Genes Dev. 19 (18): 2100-10.