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TOMM20 antibody | 4F3

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Anti Human TOMM20 Antibody, clone 4F3 thumbnail image 1 Anti Human TOMM20 Antibody, clone 4F3 thumbnail image 2 Anti Human TOMM20 Antibody, clone 4F3 thumbnail image 3
Anti Human TOMM20 Antibody, clone 4F3 gallery image 1
Western blot analysis of TOMM20 expression in HeLa using Mouse anti Human TOMM20:Azide Free (MCA4300Z)
Anti Human TOMM20 Antibody, clone 4F3 gallery image 2
Immunofluorescence showing TOMM20 in HeLa cells using Mouse anti Human TOMM20:Azide Free (MCA4300Z)
Anti Human TOMM20 Antibody, clone 4F3 gallery image 3
Staining of paraffin embedded human small intestine with Mouse anti Human TOMM20:Azide Free (MCA4300Z)

Mouse anti Human TOMM20:Preservative Free

Product Type
Monoclonal Antibody
Clone
4F3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4300Z
Datasheet
IF P* WB
SDS
0.1 mg loader

Mouse anti Human TOMM20 antibody, clone 4F3 recognizes human Mitochondrial import receptor subunit TOM20 homolog, also known as TOMM20 or Outer mitochondrial membrane receptor Tom20. TOMM20 is a 145 amino acid ~16 kDa single pass transmembrane mitochondrial protein.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-146 of human TOMM20
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q15388
Entrez Gene
TOMM20
GO Terms
GO:0051082 unfolded protein binding
GO:0016021 integral to membrane
GO:0005742 mitochondrial outer membrane translocase complex
GO:0006626 protein targeting to mitochondrion
GO:0015450 P-P-bond-hydrolysis-driven protein transmembrane transporter activity
Regulatory
For research purposes only

Applications of TOMM20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

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  • Select combined or multi-laser view to visualize the spectra
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