TNF Alpha antibody | B-C7
Mouse anti Human TNF alpha antibody, clone B-C7 recognizes both natural and bacterially producted recombinant human TNF-alpha and has an affinity of Kd = 0.5nM. Mouse anti Human TNF alpha antibody, clone B-C7 demonstrates neutralising activity against human TNF-alpha. Removal of sodium azide is recommended prior to functional assays.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Recombinant human TNF-alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag.8653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0000185 activation of MAPKKK activity
- GO:0000187 activation of MAPK activity
- GO:0001891 phagocytic cup
- GO:0002439 chronic inflammatory response to antigenic stimulus
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0009615 response to virus
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006919 activation of caspase activity
- GO:0006927 transformed cell apoptosis
- GO:0050796 regulation of insulin secretion
- GO:0042802 identical protein binding
- GO:0009651 response to salt stress
- GO:0009897 external side of plasma membrane
- GO:0010843 promoter binding
- GO:0010888 negative regulation of lipid storage
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0030730 sequestering of triglyceride
- GO:0031334 positive regulation of protein complex assembly
- GO:0031622 positive regulation of fever generation
- GO:0032582 negative regulation of gene-specific transcription
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032722 positive regulation of chemokine production
- GO:0032800 receptor biosynthetic process
- GO:0033138 positive regulation of peptidyl-serine phosphorylation
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0034116 positive regulation of heterotypic cell-cell adhesion
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043243 positive regulation of protein complex disassembly
- GO:0045071 negative regulation of viral genome replication
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045121 membrane raft
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0050715 positive regulation of cytokine secretion
- GO:0050901 leukocyte tethering or rolling
- GO:0050995 negative regulation of lipid catabolic process
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- GO:0055037 recycling endosome
- GO:0060555 induction of necroptosis by extracellular signals
- GO:0060557 positive regulation of vitamin D biosynthetic process
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0061048 negative regulation of branching involved in lung morphogenesis
- GO:0070265 necrotic cell death
- GO:0070374 positive regulation of ERK1 and ERK2 cascade
- For research purposes only
Applications of TNF Alpha antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Secondary Antibodies Available
Product Specific References
References for TNF Alpha antibody
Musey, L. et al. (2003) Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes.
J Virol. 77 (1): 291-300.
Hamacher, J. et al (2002) Tumor necrosis factor-alpha and angiostatin are mediators of endothelial cytotoxicity in bronchoalveolar lavages of patients with acute respiratory distress syndrome.
Am J Respir Crit Care Med.166: 651-6.
Hmama, Z. et al. (1992) Monocyte cytokine secretion induced by chemically-defined derivatives of Klebsiella pneumoniae.
Clin Exp Immunol. 89: 104-9.
Limb, G.A. et al. (2002) Differential expression of matrix metalloproteinases 2 and 9 by glial Müller cells: response to soluble and extracellular matrix-bound tumor necrosis factor-alpha.
Am J Pathol. 160: 1847-55.
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