TNF Alpha antibody | B-C7

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Mouse anti Human TNF Alpha

Product Type: Monoclonal Antibody
Clone: B-C7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA747	E			0.5 mg

Mouse anti Human TNF alpha antibody, clone B-C7 recognizes human tumor necrosis factor, also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. TNFα exists as a 233 amino acid membrane bound form, a single pass type II transmembrane protein which can be cleaved between residues 76 and 77 by ADAM17, also known as CD156b to yield the 157 amino acid soluble form of TNFα (UniProt: P01375).

Mouse anti Human TNF alpha antibody, clone B-C7 recognizes both natural and bacterially producted recombinant human TNF-alpha and has an affinity of Kd = 0.5nM. Mouse anti Human TNF alpha antibody, clone B-C7 demonstrates neutralising activity against human TNF-alpha. Removal of sodium azide is recommended prior to functional assays - The use of EQU003 is recommended for this purpose.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant human TNF-alpha.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag.8653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01375 Related reagents
Entrez Gene: TNF Related reagents
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0000185 activation of MAPKKK activity; GO:0000187 activation of MAPK activity; GO:0001891 phagocytic cup; GO:0002439 chronic inflammatory response to antigenic stimulus; GO:0002740 negative regulation of cytokine secretion involved in immune response; GO:0009615 response to virus; GO:0005887 integral to plasma membrane; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006919 activation of caspase activity; GO:0006927 transformed cell apoptosis; GO:0050796 regulation of insulin secretion; GO:0042802 identical protein binding; GO:0009651 response to salt stress; GO:0009897 external side of plasma membrane; GO:0010843 promoter binding; GO:0010888 negative regulation of lipid storage; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0030730 sequestering of triglyceride; GO:0031334 positive regulation of protein complex assembly; GO:0031622 positive regulation of fever generation; GO:0032582 negative regulation of gene-specific transcription; GO:0032715 negative regulation of interleukin-6 production; GO:0032722 positive regulation of chemokine production; GO:0032800 receptor biosynthetic process; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0034116 positive regulation of heterotypic cell-cell adhesion; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043193 positive regulation of gene-specific transcription; GO:0043243 positive regulation of protein complex disassembly; GO:0045071 negative regulation of viral genome replication; GO:0045080 positive regulation of chemokine biosynthetic process; GO:0045121 membrane raft; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045599 negative regulation of fat cell differentiation; GO:0045672 positive regulation of osteoclast differentiation; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050715 positive regulation of cytokine secretion; GO:0050901 leukocyte tethering or rolling; GO:0050995 negative regulation of lipid catabolic process; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051384 response to glucocorticoid stimulus; GO:0055037 recycling endosome; GO:0060555 induction of necroptosis by extracellular signals; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0061048 negative regulation of branching involved in lung morphogenesis; GO:0070265 necrotic cell death; GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory: For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

ELISA

Product Specific References

References for TNF Alpha antibody

Musey, L. et al. (2003) Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes.
J Virol. 77 (1): 291-300.
Hamacher, J. et al (2002) Tumor necrosis factor-alpha and angiostatin are mediators of endothelial cytotoxicity in bronchoalveolar lavages of patients with acute respiratory distress syndrome.
Am J Respir Crit Care Med.166: 651-6.
Hmama, Z. et al. (1992) Monocyte cytokine secretion induced by chemically-defined derivatives of Klebsiella pneumoniae.
Clin Exp Immunol. 89: 104-9.
Limb, G.A. et al. (2002) Differential expression of matrix metalloproteinases 2 and 9 by glial Müller cells: response to soluble and extracellular matrix-bound tumor necrosis factor-alpha.
Am J Pathol. 160: 1847-55.

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