TLR7 antibody | 4F4

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Anti Human TLR7 Antibody, clone 4F4 gallery image 1 — Western blot analysis of TLR7 expression against recombinant TLR7 (immunogen) using Mouse anti Human TLR7 (MCA2702GA)

Mouse anti Human TLR7

Product Type: Monoclonal Antibody
Clone: 4F4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2702GA	E WB			0.1 mg

Mouse anti Human TLR7 antibody, clone 4F4 recognizes human TLR7 (toll-like receptor 7), a 121 kDa type I transmembrane protein belonging to the toll-like receptor (TLR) family. TLR7is predominantly expressed in the spleen, lung and placenta (Chuang et al. 2000).

TLRs are highly conserved proteins which play a key role in activation of innate immunity, through the recognition of distinct molecular patterns expressed by infectious agents. TLR7 recognizes and interacts with single-stranded RNA (ssRNA) derived from viruses such as influenza , HIV-1 (human immunodeficiency virus type I) and Sendai, and some siRNAs, resulting in the activation of the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway, and the production of proinflammatory signaling molecules (Diebold et al. 2004)

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
Immunogen: Recombinant human TLR7 (amino acids 451-500) purified from Escherichia coli.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q9NYK1 Related reagents
Entrez Gene: TLR7 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0003725 double-stranded RNA binding; GO:0003727 single-stranded RNA binding; GO:0005764 lysosome; GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005789 endoplasmic reticulum membrane; GO:0006954 inflammatory response; GO:0007252 I-kappaB phosphorylation; GO:0010008 endosome membrane; GO:0032009 early phagosome; GO:0032722 positive regulation of chemokine production; GO:0032757 positive regulation of interleukin-8 production; GO:0035197 siRNA binding; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045087 innate immune response; GO:0045356 positive regulation of interferon-alpha biosynthetic process; GO:0045359 positive regulation of interferon-beta biosynthetic process; GO:0045416 positive regulation of interleukin-8 biosynthetic process; GO:0050729 positive regulation of inflammatory response; GO:0051607 defense response to virus
Regulatory: For research purposes only

Applications of TLR7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting		1/1000	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: MCA2702GA detects a band of approximately 121 kDa in Ramos cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F