TLR7 antibody | 4F4

Mouse anti Human TLR7

Product Type
Monoclonal Antibody
Clone
4F4
Isotype
IgG1
Specificity
TLR7

Product Code Applications Pack Size List Price Your Price Qty
MCA2702GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human TLR7 antibody, clone 4F4 recognizes human TLR7 (toll-like receptor 7), a 121 kDa type I transmembrane protein belonging to the toll-like receptor (TLR) family. TLR7is predominantly expressed in the spleen, lung and placenta (Chuang et al. 2000).

TLRs are highly conserved proteins which play a key role in activation of innate immunity, through the recognition of distinct molecular patterns expressed by infectious agents. TLR7 recognizes and interacts with single-stranded RNA (ssRNA) derived from viruses such as influenza , HIV-1 (human immunodeficiency virus type I) and Sendai, and some siRNAs, resulting in the activation of the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway, and the production of proinflammatory signaling molecules (Diebold et al. 2004).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
10% Glycerol
Immunogen
Recombinant human TLR7 (amino acids 451-500) purified from Escherichia coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2702GA detects a band of approximately 121 kDa in Ramos cell lysates.

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Further Reading

  1. Kawai, T. & Akira, S. (2006) TLR signaling.
    Cell Death Differ. 13 (5): 816-25.

RRID
AB_2256006
UniProt
Q9NYK1
Entrez Gene
TLR7
GO Terms
GO:0005886 plasma membrane
GO:0003725 double-stranded RNA binding
GO:0003727 single-stranded RNA binding
GO:0005764 lysosome
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0010008 endosome membrane
GO:0032009 early phagosome
GO:0032722 positive regulation of chemokine production
GO:0032757 positive regulation of interleukin-8 production
GO:0035197 siRNA binding
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0045416 positive regulation of interleukin-8 biosynthetic process
GO:0050729 positive regulation of inflammatory response
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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