TIM-4 antibody | rAB04-3G2

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Mouse anti Human TIM-4

Product Type: Monoclonal Antibody
Clone: rAB04-3G2
Isotype: IgG1
Specificity: TIM-4

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Product Code Applications Pack Size List Price Your Price Qty

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TZA096GA Datasheet SDS SDS	F	0.1 mg		Log in
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Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 1

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 2

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 3

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 4

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 5

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 6

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 7

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 1 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBUV795 (**TZA096SBUV795**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 2 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBV515 (**TZA096SBV515**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 3 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBV610 (**TZA096SBV610**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 4 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBB580 (**TZA096SBB580**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 5 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBB615 (**TZA096SBB615**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 6 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBY720 (**TZA096SBY720**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 7 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBUV445 (**TZA096SBUV445**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

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Mouse anti Human TIM-4 antibody, clone rAB04-3G2, is a recombinant monoclonal antibody that recognizes TIM-4, also known as SMUCKLER, T-cell immunoglobulin and mucin domain-containing protein 4 or TIMD-4.

TIM-4 is a ~ 42 kDa type I membrane protein which is preferentially expressed on macrophages, mature dendritic cells, peritoneal B1 cells and invariant natural killer T (iNKT) cells. TIM-4 is the ligand of TIM-1, and binding leads to T-cell proliferation and cytokine production.

Polymorphisms in the TIM-4 gene may be associated with a predisposition to asthma. TIM-4 is expressed in various tumor cells, including lung cancer, colorectal cancer, and certain sarcomas. It can contribute to tumor growth and immune evasion by modulating the tumor microenvironment and promoting angiogenesis.

Target Species: Human
Product Form: Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: CHO cells
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant TIM-4 Glu25-Gln314 expressed by mammalian cells
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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