Tenascin antibody | T2H5

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Mouse anti Human Tenascin

Product Type
Monoclonal Antibody
Clone
T2H5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8640-0208 C E IP P* WB 0.2 mg
Mouse anti Human Tenascin antibody, clone T2H5 recognizes tenascin in both normal and hyperproliferative (also neoplastic) tissues.

Tenascin has been implicated in the guidance of migrating neurons and axons during development and neuronal regeneration. It promotes cortical neuron outgrowths and is an important component of the wound matrix and scar formation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified protein from human mammary tumour.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P24821 Related reagents
Entrez Gene
TNC Related reagents
GO Terms
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0009611 response to wounding
GO:0045545 syndecan binding
Regulatory
For research purposes only

Applications of Tenascin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/2000
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 Staining may be enhanced using trypsin digestion or heat mediated antigen retrieval using sodium citrate buffer pH 6.0.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
This product is suitable for use in direct ELISA applications, 8640-0502 may be used as a standard.
Histology Positive Control Tissue
Human tonsil.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Schalkwijk, J. et al. (1991) Tenascin expression in human dermis is related to epidermal proliferation.
    Am J Pathol. 139 (5): 1143-50.

References for Tenascin antibody

  1. Verstraeten, A.A. et al. (1992) Tenascin expression in basal cell carcinoma.
    Br J Dermatol. 127 (6): 571-4.
  2. Le Poole, I.C. et al. (1997) Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion.
    Br J Dermatol. 137 (2): 171-8.
  3. Milz, S. et al. (2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.
    J Anat. 211: 1-7.
  4. Päiväniemi, O, et al. (2006) Tenascin is expressed in post-transplant obliterative bronchiolitis.
    Transplant Proc. 38: 2694-6.
  5. Nakamura, Y. et al. (2004) Ets-1 regulates TNF-alpha-induced matrix metalloproteinase-9 and tenascin expression in primary bronchial fibroblasts.
    J Immunol. 172: 1945-52.
  6. Milz, S. et al. (2002) Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.
    Invest Ophthalmol Vis Sci. 43: 1330-4.