TCR Alpha antibody | 3A8

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Mouse anti Human TCR Alpha Chain

Product Type
Monoclonal Antibody
Clone
3A8
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA987T C IP 50 µg
MCA987 C IP 0.1 mg
Mouse anti Human TCR alpha Chain antibody, clone 3A8 reacts with the alpha chain constant region found on all alpha/beta TCR bearing T-lymphocytes. It reacts with 92-98% of all CD3 + peripheral blood lymphocytes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Pack Size: 50 µg
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.1 mg
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01848 Related reagents
Entrez Gene
TRAC Related reagents
GO Terms
GO:0004872 receptor activity
GO:0042605 peptide antigen binding
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
GO:0031295 T cell costimulation
GO:0042287 MHC protein binding
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of TCR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for TCR Alpha antibody

  1. Henry, L. et al. (1989) Two distinct immunogenic epitopes on the alpha chain of human T cell antigen receptor.
    Hybridoma. 8 (6): 577-88.
  2. Koning, F. et al. (1988) The biosynthesis and assembly of T cell receptor alpha- and beta-chains with the CD3 complex.
    J Immunol. 140 (9): 3126-34.
  3. Minami, Y. et al. (1987) Building a multichain receptor: synthesis, degradation, and assembly of the T-cell antigen receptor.
    Proc Natl Acad Sci U S A. 84 (9): 2688-92.
  4. van Dongen, J.J. et al. (1987) T cell receptor-CD3 complex during early T cell differentiation. Analysis of immature T cell acute lymphoblastic leukemias (T-ALL) at DNA, RNA, and cell membrane level.
    J Immunol. 138 (4): 1260-9.