SQSTM1 antibody | 2C11

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Mouse anti Human SQSTM1:Preservative Free

Product Type
Monoclonal Antibody
Clone
2C11
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4265Z IF WB 0.1 mg
Mouse anti Human SQSTM1 antibody, clone 2C11 recognizes human SQSTM1, also known as Sequestosome-1, p62 or EBIAP. SQSTM1 is a ~62kDa ubiquitously expressed molecule bearing an N-terminal OPR domain which mediates homooligomerization and interactions with other proteins. SQSTM1 also bears a C-terminal UBA domain, responsible for binding to polyubiquitinated substrates.

SQSTM1 is involved in the autophagic degradation of polyubiquitinated proteins (Wei et al. 2013) and in bone remodelling through activation of of osteoclasts. Mutations in the SQSTM1 gene have been implicated in Paget's disease of bone (Laurin et al. 2002)

Mouse anti human SQSTM1, clone 2C11 has been used successfully for the identification of SQSTM1 in Hela cells by western blotting.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1 - 441 of human SQSTM1.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13501 Related reagents
Entrez Gene
SQSTM1 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0008104 protein localization
GO:0005080 protein kinase C binding
GO:0005654 nucleoplasm
GO:0005770 late endosome
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006916 anti-apoptosis
GO:0030971 receptor tyrosine kinase binding
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016197 endosome transport
GO:0016236 macroautophagy
GO:0030154 cell differentiation
GO:0042169 SH2 domain binding
GO:0043122 regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of SQSTM1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F