Sphingosine 1- Phosphate Receptor 1 antibody | 2B9

Mouse anti Human Sphingosine 1- Phosphate Receptor 1

Product Type
Monoclonal Antibody
Clone
2B9
Isotype
IgG2a
Specificity
Sphingosine 1- Phosphate Receptor 1

Product Code Applications Pack Size List Price Your Price Qty
MCA5960GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Sphingosine 1-Phosphate Receptor 1, clone 2B9 recognizes S1P1, otherwise known as CD363/EDG-1, a bioactive lysophospholipid and inducible member of the G-protein coupled receptor superfamily (GPCR) which plays an important role in a variety of cellular processes.

During inflammatory diseases such as atherosclerosis the phenotype switching of activated macrophages is influenced by the presence of particular cytokines within the microenvironment. These cells have been characterized as classically activated M1 macrophages, in response to pro-inflammatory molecules such as interferon gamma and lipopolysaccharide, or as alternatively activated M2 macrophages, in response to anti-inflammatory cytokines such as interleukin-4. Activation of S1P1 through its ligand S1P (Sphingosine 1-Phosphate) has been identified as an important promoter of anti-inflammatory M2 macrophages (Hughes et al. 2008).

Apoptotic cells can also influence the pro-versus anti-inflammatory macrophage response, and S1P acts as a positive regulator for anti-apoptotic/pro-survival responses by binding to S1P1. Growing evidence suggests that the expression of S1P1 on macrophages may convey protection against apoptosis, at least in part, having implications for the pathogenesis of inflammatory diseases and cancer.

Deviation in sphingosine 1-phosphate (S1P) signaling is important in many types of cancer. Analysis of S1P1 and SK1 receptors during the development of tamoxifen resistance in Estrogen Receptor (ER) breast cancer patients concurs that the expression levels of S1P1 correlates with tumor size and Progesterone Receptor (PR) status and that high S1P1 membrane expression is associated with shorter time to recurrence.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Immunofluorescence 1/500
Immunoprecipitation 1/300
Western Blotting 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
List Price Your Price
loader
Description Human anti Mouse IgG2a:HRP
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG2a Negative Control

Synonyms
CD363
S1P1
UniProt
P21453
Entrez Gene
S1PR1
GO Terms
GO:0005886 plasma membrane
GO:0001619 lysosphingolipid and lysophosphatidic acid receptor activity
GO:0007155 cell adhesion
GO:0016021 integral to membrane
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products