Sphingosine 1- Phosphate Receptor 1 antibody | 2B9
lane 1: CH0-S1P1-GFP
Lane 2: Control with no primary antibody
Lane 3: CHO-S1P1-GFP solubized with NP-40
Lane 4: Immunoprecipitated sample using Mouse anti Human Sphingosine 1-phosphate receptor 1(MCA5960GA).
Samples were probed using a Rabbit anti GFP coupled to Horseradish peroxidase and visualized using chemiluminescence.
Lane 1: CHO-K1 cell membranes (negative control)
Lane 2: CHO-S1P1-GFP stable transfectant
Lane 3: Huvec cell membranes
The GFP-S1P1 transfectant migrates at an apparent MW of ~70kDa whilst the native S1P1 expressed on HUVEC cells migrates at an apparent MW of ~43kDa.
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FLA IF IP WB ResetMouse anti Human Sphingosine 1- Phosphate Receptor 1
- Product Type
- Monoclonal Antibody
- Clone
- 2B9
- Isotype
- IgG2a
- Specificity
- Sphingosine 1- Phosphate Receptor 1
Antibody Quality and 
Unrivaled 
| Mouse anti Human Sphingosine 1-Phosphate Receptor 1, clone 2B9 recognizes S1P1, otherwise known as CD363/EDG-1, a bioactive lysophospholipid and inducible member of the G-protein coupled receptor superfamily (GPCR) which plays an important role in a variety of cellular processes. During inflammatory diseases such as atherosclerosis the phenotype switching of activated macrophages is influenced by the presence of particular cytokines within the microenvironment. These cells have been characterized as classically activated M1 macrophages, in response to pro-inflammatory molecules such as interferon gamma and lipopolysaccharide, or as alternatively activated M2 macrophages, in response to anti-inflammatory cytokines such as interleukin-4. Activation of S1P1 through its ligand S1P (Sphingosine 1-Phosphate) has been identified as an important promoter of anti-inflammatory M2 macrophages (Hughes et al. 2008). Apoptotic cells can also influence the pro-versus anti-inflammatory macrophage response, and S1P acts as a positive regulator for anti-apoptotic/pro-survival responses by binding to S1P1. Growing evidence suggests that the expression of S1P1 on macrophages may convey protection against apoptosis, at least in part, having implications for the pathogenesis of inflammatory diseases and cancer. Deviation in sphingosine 1-phosphate (S1P) signaling is important in many types of cancer. Analysis of S1P1 and SK1 receptors during the development of tamoxifen resistance in Estrogen Receptor (ER) breast cancer patients concurs that the expression levels of S1P1 correlates with tumor size and Progesterone Receptor (PR) status and that high S1P1 membrane expression is associated with shorter time to recurrence. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | |
| Immunofluorescence | |
1/500 | |
| Immunoprecipitation | |
1/300 | |
| Western Blotting | |
1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
- Synonyms
- CD363
- S1P1
- UniProt
- P21453
- Entrez Gene
- S1PR1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001619 lysosphingolipid and lysophosphatidic acid receptor activity
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
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