Siglec-7 antibody | S7.7

Mouse anti Human Siglec-7

Product Type
Monoclonal Antibody
Clone
S7.7
Isotype
IgG1
Specificity
Siglec-7

Product Code Applications Pack Size List Price Your Price Qty
MCA5782GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP WB 0.1 mg loader
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loader
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Mouse anti Human Siglec-7 antibody, cloneS7.7 recognizes human Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells.

Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis.

Mouse anti Human Siglec-7 antibody, cloneS7.7 is a blocking antibody (Avril et al. 2006), and has been shown to recognize Siglec-7 in Rhesus monkey (Jaroenpool et al. 2007).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Siglec-7 3T3 transfected cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice, were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/200
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.
Western Blotting
MCA5782GA detects a band of approximately 65kDa using Siglec-7 transfected CHO cells see Nicoll, G. et al. for details.

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Description Mouse IgG1 Negative Control

References for Siglec-7 antibody

  1. Nicoll, G. et al. (1999) Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes.
    J Biol Chem. 274 (48): 34089-95.
  2. Jaroenpool, J. et al. (2007) Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates.
    Cell Immunol. 250 (1-2): 91-104.
  3. Avril T et al. (2006) Sialic acid-binding immunoglobulin-like lectin 7 mediates selective recognition of sialylated glycans expressed on Campylobacter jejuni lipooligosaccharides.
    Infect Immun. 74 (7): 4133-41.
  4. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
    Proc Natl Acad Sci U S A. 103 (20): 7765-70.

Synonyms
CD328
UniProt
Q9Y286
Entrez Gene
SIGLEC7
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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