Siglec-5/Siglec-14 antibody | 1A5

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Mouse anti Human Siglec-5/Siglec-14:FITC

Mouse anti Human Siglec-5/Siglec-14

Mouse anti Human Siglec-5/Siglec-14:RPE

Product Type
Monoclonal Antibody
Clone
1A5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5787F F 0.1 mg
MCA5787GA E F IF IP 0.1 mg
MCA5787PE F 100 Tests
Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5 recognizes human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage.

Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5, is one of several Siglec-5 antibodies which also recognises human Siglec-14 (Angata et al. 2006). Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.

Siglec-5 is also related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation.

Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5 cross reacts with Chimpanzee (Jaroenpool et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
O15389 Related reagents
Q08ET2 Related reagents
Entrez Gene
SIGLEC5 Related reagents
SIGLEC14 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0005886 plasma membrane
GO:0005515 protein binding
Regulatory
For research purposes only

Applications of Siglec-5/Siglec-14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
ELISA
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Immunoprecipitation

Product Specific References

References for Siglec-5/Siglec-14 antibody

  1. Cornish, A.L. et al. (1998) Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.
    Blood. 92 (6): 2123-32.
  2. Avril, T. et al. (2005) Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.
    J Biol Chem. 280 (20): 19843-51.
  3. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
    Proc Natl Acad Sci U S A. 103 (20): 7765-70.
  4. Jaroenpool, J. et al. (2007) Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates.
    Cell Immunol. 250 (1-2): 91-104.
  5. Angata, T. et al. (2006) Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
    FASEB J. 20: 1964-1973.

Further Reading

  1. Crocker, P.R. (2005) Siglecs in innate immunity.
    Curr Opin Pharmacol. 5 (4): 431-7.