Siglec-10 antibody | 5G6
Mouse anti Human Siglec-10
- Product Type
- Monoclonal Antibody
- Clone
- 5G6
- Isotype
- IgG1
- Specificity
- Siglec-10
Antibody Quality and Performance Guarantee
| Mouse anti Human Siglec-10 antibody, clone 5G6 recognizes human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, (Kivi et al. 2009). This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9 (Munday et al. 2001). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant human Siglec-10, fused with the Fc region of human IgG
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Siglec-10 antibody
-
Munday, J. et al. (2001) Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.
Biochem J. 355 (Pt 2): 489-97. -
Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
Proc Natl Acad Sci U S A. 103 (20): 7765-70. -
Kivi, E. et al. (2009) Human Siglec-10 can bind to vascular adhesion protein-1 and serves as its substrate.
Blood. 114 (26): 5385-92. -
Ju, B. et al. (2022) Elevated CD19+Siglec-10+ B cell levels are correlated with systemic lupus erythematosus disease activity.
Int Immunopharmacol. 102: 108403.
Further Reading
-
Crocker, P.R. (2005) Siglecs in innate immunity.
Curr Opin Pharmacol. 5 (4): 431-7.
- Synonyms
- Sialic ACID-BINDING IG-LIKE Lectin 10
- UniProt
- Q96LC7
- Entrez Gene
- SIGLEC10
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005529 sugar binding
MCA5783GA
152792
155873
161756
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