Siglec-10 antibody | 5G6

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Anti Human Siglec-10 Antibody, clone 5G6 gallery image 1 — Siglec-10 tranfected CHO cells stained with Mouse anti Human siglec-10 (**MCA5783GA**) followed by Rabbit F(ab')₂ anti Mouse IgG:FITC (STAR9B).

Mouse anti Human Siglec-10:FITC

Mouse anti Human Siglec-10

Mouse anti Human Siglec-10:RPE

Product Type: Monoclonal Antibody
Clone: 5G6
Isotype: IgG1

Product Code	Applications	Pack Size
MCA5783F	F	0.1 mg
MCA5783GA	F	0.1 mg
MCA5783PE	F	100 Tests

Mouse anti Human Siglec-10 antibody, clone 5G6 recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, (Kivi et al. 2009). This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9 (Munday et al. 2001).

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: Recombinant human Siglec-10, fused with the Fc region of human IgG
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: Q96LC7 Related reagents
Entrez Gene: SIGLEC10 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0005529 sugar binding
Regulatory: For research purposes only

Applications of Siglec-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Flow Cytometry	Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for Siglec-10 antibody

Munday, J. et al. (2001) Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.
Biochem J. 355 (Pt 2): 489-97.
Kivi, E. et al. (2009) Human Siglec-10 can bind to vascular adhesion protein-1 and serves as its substrate.
Blood. 114 (26): 5385-92.
Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
Proc Natl Acad Sci U S A. 103 (20): 7765-70.