Siglec-10 antibody | 5G6

Mouse anti Human Siglec-10

Product Type
Monoclonal Antibody
Clone
5G6
Isotype
IgG1
Specificity
Siglec-10

Product Code Applications Pack Size List Price Your Price Qty
MCA5783GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human Siglec-10 antibody, clone 5G6 recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, (Kivi et al. 2009). This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9 (Munday et al. 2001).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant human Siglec-10, fused with the Fc region of human IgG
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.

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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for Siglec-10 antibody

  1. Munday, J. et al. (2001) Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.
    Biochem J. 355 (Pt 2): 489-97.
  2. Kivi, E. et al. (2009) Human Siglec-10 can bind to vascular adhesion protein-1 and serves as its substrate.
    Blood. 114 (26): 5385-92.
  3. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
    Proc Natl Acad Sci U S A. 103 (20): 7765-70.
  4. Ju, B. et al. (2022) Elevated CD19+Siglec-10+ B cell levels are correlated with systemic lupus erythematosus disease activity.
    Int Immunopharmacol. 102: 108403.

Further Reading

  1. Crocker, P.R. (2005) Siglecs in innate immunity.
    Curr Opin Pharmacol. 5 (4): 431-7.

Synonyms
Sialic ACID-BINDING IG-LIKE Lectin 10
UniProt
Q96LC7
Entrez Gene
SIGLEC10
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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