Serum Amyloid A antibody | SAA19

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Mouse anti Human Serum Amyloid A

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6030GA E WB 0.1 mg
Mouse anti Human serum amyloid A, clone SAA19 recognizes the human serum amyloid A (SAA) protein, an acute phase protein belonging to the SAA family which is secreted into the plasma by the liver following injury or infection.

SAA is a widely used marker of inflamation as elevated levels of SAA may be detected in the blood of the individual following infection or tissue trauma.

Serum amyloid A is highly conserved between species with human SAA being similar in structure to SAA in other species. Studies have been undertaken that demonstrate monoclonal antibodies raised against human SAA can be used to develop assays for measurement of SAA in domestic animals (Christensen, M, et al.).

In addition to human SAA, clone SAA19 recognises canine, equine and feline SAA. Structurally canine, feline and equine SAA differ from human SAA in that they contain an 8 aa insert in the central region of the sequence, human SSA being 104 aa, equine SAA 110 aa, while canine and feline SAA are 111 aa.

Clone SAA19 may be use as a capture reagent along with clone VSA34 (MCA6031GA) as the detection reagent in the development of immunoassays to detect SAA in dogs, cats and horses along with recombinant SAA (for canine, product code PDP006, for feline, product code PFP003 and for equine, product code PEP006) as standards.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Human Serum Amyloid A (SAA)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
18 months from date of despatch

More Information

P19708 Related reagents
P19857 Related reagents
P19707 Related reagents
P0DJI8 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0034364 high-density lipoprotein particle
For research purposes only

Applications of Serum Amyloid A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA, together with MCA6031GA, as the detection reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Dog Serum Amyloid A MCA6031GA 0.1 mg E WB
Recombinant Dog Serum Amyloid A PDP006 0.1 mg E
Recombinant Horse Serum Amyloid A PEP006 0.1 mg E
Recombinant CAT Serum Amyloid A PFP003 0.1 mg E

Product Specific References

Further Reading

  1. Christensen, M et al. (2012) Evaluation of an automated assay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline, and equine SAA.
    Vet J. 194: 332-7.
  2. Jitpean, S. et al. (2014) Increased concentrations of Serum amyloid A in dogs with sepsis caused by pyometra.
    BMC Vet Res. 10: 273.
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