Mouse anti Dog serum amyloid A, clone VSA34, specifically recognizes the canine serum amyloid A (SAA) protein, an acute phase protein belonging to the SAA family. SAA is secreted into the plasma by the liver following injury or infection.
SAA is a widely used marker of inflamation as elevated levels of SAA may be detected in the blood of the animal following infection or tissue trauma.
Serum amyloid A is highly conserved between species with canine SAA being similar in structure to SAA in other species. Clone VSA34 recognises both equine and feline SAA. Structurally canine, feline and equine SAA differ from human SAA in that they contain an 8 aa insert in the central region of the sequence, human SSA being 104 aa, equine SAA 110 aa while canine and feline have 111 aa.
Clone VSA34 is specifically raised against dog, but may be used to detect SAA in cats and horses. Clone VSA34 may be use as a detection reagent along with clone SAA19 (MCA6030GA) as the capture reagent in the development of immunoassays to detect SAA in dogs, cats and horses along with recombinant SAA (for canine product code PDP006, for feline, product code PFP003 and equine, product code PEP006) as a standard.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A
0.09% Sodium Azide (NaN3)
Canine Serum Amyloid A (SAA)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when either conjugated to HRP, or with appropriately conjugated secondary antibody, together with MCA6030GA as the capture reagent.