Serum Amyloid A Antibody | VSA34

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  • Mouse anti Dog Serum Amyloid A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    VSA34
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6031GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA6031GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Dog serum amyloid A, clone VSA34, specifically recognizes the canine serum amyloid A (SAA) protein, an acute phase protein belonging to the SAA family. SAA is secreted into the plasma by the liver following injury or infection.

      SAA is a widely used marker of inflamation as elevated levels of SAA may be detected in the blood of the animal following infection or tissue trauma.

      Serum amyloid A is highly conserved between species with canine SAA being similar in structure to SAA in other species. Clone VSA34 recognises both equine and feline SAA. Structurally canine, feline and equine SAA differ from human SAA in that they contain an 8 aa insert in the central region of the sequence, human SSA being 104 aa, equine SAA 110 aa while canine and feline have 111 aa.

      Clone VSA34 is specifically raised against dog, but may be used to detect SAA in cats and horses. Clone VSA34 may be use as a detection reagent along with clone SAA19 (MCA6030GA) as the capture reagent in the development of immunoassays to detect SAA in dogs, cats and horses along with recombinant SAA (for canine product code PDP006, for feline, product code PFP003 and equine, product code PEP006) as a standard.
    • Intended Use
    • Target Species
      Dog
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Catyes
      Horseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Canine Serum Amyloid A (SAA)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      acute-phase response
      high-density lipoprotein particle
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA, when either conjugated to HRP, or with appropriately conjugated secondary antibody, together with MCA6030GA as the capture reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Serum Amyloid A Antibody Formats

    Formats Clone Applications Sizes available
    Serum Amyloid A Antibody : Purified VSA34 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Dog Serum Amyloid AMCA6031GA0.1 mgE, WB
          MCA6031GA
          Recombinant Dog Serum Amyloid APDP0060.1 mgE
          PDP006
          Recombinant Horse Serum Amyloid APEP0060.1 mgE
          PEP006
          Recombinant Cat Serum Amyloid APFP0030.1 mgE
          PFP003

          Recommended Positive Controls

            Histology Controls

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