Recombinant Dog serum amyloid A (SAA) is a 121 aa recombinant protein produced in a eukaryotic expression system, which includes a proprietary tag at the N-terminus.
Serum amyloid A proteins are a family of acute phase proteins, produced primarily by the liver, highly conserved in vertebrate species, and used as markers of inflammation.
Canine SAA, as with SAA in other species, is present in different isoforms, this recombinant protein corresponds to isoform 1 (SAA1). Canine SAA is composed of 111 amino acids and differs from human SAA by having an 8 aa sequence insert in the central region of the sequence.
With similar biochemical properties to the native canine SAA, this reagent may be used as a standard in immunoassays for dog serum amyloid A.
Purified recombinant protein - lyophilized
Reconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified by affinity and reversed-phase chromatography
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at -20oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.