S100A4 antibody | 1F12-1G7
Mouse anti Human S100A4:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1F12-1G7
- Isotype
- IgG1
- Specificity
- S100A4
Mouse anti Human S100A4 antibody, clone 1F12-1G7 recognizes human S100A4, also known as S100 calcium-binding protein A4, a member of the S-100 protein family containing two EF-hand calcium-binding domains. S100A4 may play a role in motility, invasion and tubulin polymerization and is also involved in tumor metastasis. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein with a GST tag corresponding to amino acids 1 - 102 of human S100A4
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence 1 | 0.1 | 10 ug/ml | |
Western Blotting | 0.1 | 10 ug/ml |
- 1 This product requires fixation of cells for 15 minutes. 4 % paraformaldehyde solution (NaOH in PBS) pH 7.4 is recommended for this purpose.
- Western Blotting
- MCA3436Z detects a band of approximately 10 kDa in HeLa cell lysates
- RRID
- AB_2183771
- UniProt
- P26447
- Entrez Gene
- S100A4
- GO Terms
- GO:0005509 calcium ion binding
- GO:0001837 epithelial to mesenchymal transition
- GO:0005634 nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0048471 perinuclear region of cytoplasm
- GO:0050786 RAGE receptor binding
MCA3436Z
151203 171002If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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