RNA Pol II CTD (pSer2/pSer5) antibody | 1A12G10
Mouse anti Human RNA Pol II CTD (pSer2/pSer5)
- Product Type
- Monoclonal Antibody
- Clone
- 1A12G10
- Isotype
- IgG1
- Specificity
- RNA Pol II CTD (pSer2/pSer5)
Antibody Quality and Performance Guarantee
| Mouse anti Human RNA pol II CTD (pSer2/pSer5) antibody, clone 1A12G10 recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 2 or serine 5 of the carboxyl-terminal repeat domain. RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999) (Sims et al. 2004).The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle (Phatnani and Greenleaf, 2006). Repeats phosphorylated on both serine 2 and serine 5 are required for optimal binding of the SRI domain of the methyl transerase SET2 to RNA pol II (Rebehmed et al. 2014). Wide species cross-reactivity is expected from Mouse anti Human RNA pol II CTD (pSer2/pSer5), clone 1A12G10 based on sequence. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A synthetic peptide containing phosphorylated serine 2 and phosphorylated serine 5 of the CTD of human RNA polymerase II
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Chromatin Immunoprecipitation |  |
5 µg per ChIP | 10 µg per ChIP |
| Immunofluorescence | |
5 µg/ml | |
| Western Blotting | |
0.5 µl/ml | 2 µl/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- RPB1
- UniProt
- P24928
- Entrez Gene
- POLR2A
- GO Terms
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0003968 RNA-directed RNA polymerase activity
- GO:0046872 metal ion binding
- GO:0005665 DNA-directed RNA polymerase II, core complex
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0006367 transcription initiation from RNA polymerase II promoter
- View More GO Terms
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006370 mRNA capping
- GO:0016032 viral reproduction
- GO:0031625 ubiquitin protein ligase binding
- GO:0050434 positive regulation of viral transcription
MCA6374
160852
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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