Prolactin antibody | INN-hPRL-1

Mouse anti Human Prolactin (Epitope C)

Product Type
Monoclonal Antibody
Clone
INN-hPRL-1
Isotype
IgG1
Specificity
Prolactin
Region
(EPITOPE c)

Product Code Applications Pack Size List Price Your Price Qty
MCA712
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P * R 0.5 mg loader
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loader
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Mouse anti Human Prolactin antibody, clone INN-hPRL-1 recognises human prolactin, also known as luteotropic hormone or luteotropin, binding to epitope 'c' as determined by a competitive binding assay (Staindl et al. 1987). Prolactin is a 199 amnio acid ~24 kDa secreted anterior pituitary hormone acting to promote lactation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/200
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1 1/500 1/1000
Radioimmunoassays
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Pituitary

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References for Prolactin antibody

  1. Staindl, B. et al. (1987) Monoclonal antibodies against human, bovine and rat prolactin: epitope mapping of human prolactin and development of a two-site immunoradiometric assay.
    J Endocrinol. 114 (2): 311-8.
  2. Toth, B. et al. (2009) Insulin and leptin receptors as possible new candidates for endocrine control in normal and disturbed human pregnancy.
    Mol Hum Reprod. 15:231-9.
  3. Ye, Y. et al. (2018) Prostaglandin E2 receptor 3 signaling is induced in placentas with unexplained recurrent pregnancy losses.
    Endocr Connect. 7 (5): 749-761.

Immunofluorescence

Synonyms
PRL
RRID
AB_322383
UniProt
P01236
Entrez Gene
PRL
GO Terms
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005148 prolactin receptor binding
GO:0005179 hormone activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0007565 female pregnancy
GO:0007595 lactation
GO:0008283 cell proliferation
GO:0040014 regulation of multicellular organism growth
GO:0046427 positive regulation of JAK-STAT cascade
View more products with PROLACTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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