Prolactin antibody | INN-hPRL-1
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Mouse anti Human Prolactin (Epitope C)
- Product Type
- Monoclonal Antibody
- Clone
- INN-hPRL-1
- Isotype
- IgG1
- Specificity
- Prolactin
- Region
- (EPITOPE c)
Antibody Quality and Performance Guarantee
| Mouse anti Human Prolactin antibody, clone INN-hPRL-1 recognises human prolactin, also known as luteotropic hormone or luteotropin, binding to epitope 'c' as determined by a competitive binding assay (Staindl et al. 1987). Prolactin is a 199 amnio acid ~24 kDa secreted anterior pituitary hormone acting to promote lactation. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20 | 1/200 |
| Immunohistology - Frozen | |
1/500 | 1/1000 |
| Immunohistology - Paraffin 1 | |
1/500 | 1/1000 |
| Radioimmunoassays | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Pituitary
References for Prolactin antibody
-
Staindl, B. et al. (1987) Monoclonal antibodies against human, bovine and rat prolactin: epitope mapping of human prolactin and development of a two-site immunoradiometric assay.
J Endocrinol. 114 (2): 311-8. -
Toth, B. et al. (2009) Insulin and leptin receptors as possible new candidates for endocrine control in normal and disturbed human pregnancy.
Mol Hum Reprod. 15:231-9. -
Toth, B. et al. (2010) Placental interleukin-15 expression in recurrent miscarriage.
Am J Reprod Immunol. 64 (6): 402-10. -
Ye, Y. et al. (2018) Prostaglandin E2 receptor 3 signaling is induced in placentas with unexplained recurrent pregnancy losses.
Endocr Connect. 7 (5): 749-761.
- Synonyms
- PRL
- RRID
- AB_322383
- UniProt
- P01236
- Entrez Gene
- PRL
- GO Terms
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005148 prolactin receptor binding
- GO:0005179 hormone activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0007565 female pregnancy
- GO:0007595 lactation
- GO:0008283 cell proliferation
- GO:0040014 regulation of multicellular organism growth
- View More GO Terms
- GO:0046427 positive regulation of JAK-STAT cascade
MCA712
0414
152014
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with PROLACTIN specificity
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