Prolactin antibody | C01-2B2

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Mouse anti Human Prolactin

Product Type
Monoclonal Antibody
Clone
C01-2B2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6200GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human prolactin antibody, clone C01-2B2, recognizes prolactin a peptide hormone expressed by many cell types including the pituitary gland, decidua, ovary, prostate, mammary gland, adipose tissue, brain and immune cells. Of the immune cells, prolactin is secreted by dentritic cells, thymocytes, T cells, B cells, monocytes and macrophages (Borba et al. 2018). Prolactin now has a reported >300 separate biological activities, since it was first discovered as a stimulator of crop milk in pigeons and a promotor of lactation in rabbits (Freeman et al. 2000). In the immune system prolactin acts as a cytokine. In an example, prolactin is able to regulate innate and adaptive immune responses through the maturation of CD4- CD8- thymocytes to CD4+ CD8+ T cells via IL-2 receptor expression. Prolactin is also able to stimulate the development of antigen presenting cells expressing major histocompatibility complex class II (Borba et al. 2018). Where patients have an abnormally high level of circulating prolactin (hyperprolactinemia) this can result in the impairment of B cell clonal deletion, deregulation or receptor editing, as well as the diminishment of the threshold for B cell activation, promoting auto-reactivity (Borba et al. 2018).

The purified Mouse anti Human prolactin antibody, clone C01-2B2 (MCA6200GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human prolactin antibody, clone C03-3E9 (MCA6201B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human prolactin (Leu29-Cys227)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P01236
Entrez Gene
PRL
GO Terms
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005148 prolactin receptor binding
GO:0005179 hormone activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0007565 female pregnancy
GO:0007595 lactation
GO:0008283 cell proliferation
GO:0040014 regulation of multicellular organism growth
GO:0046427 positive regulation of JAK-STAT cascade
Regulatory
For research purposes only

Applications of Prolactin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Prolactin:Biotin MCA6201B E 0.1 mg loader

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