Parkin antibody | 1H4
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WB IF ResetMouse anti Human Parkin:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1H4
- Isotype
- IgG3
- Specificity
- Parkin
Antibody Quality and 
Unrivaled 
| Mouse anti Human Parkin antibody, clone 1H4 recognizes human E3 ubiquitin-protein ligase parkin, also known as Parkin or Parkinson juvenile disease protein 2. Parkin is a 465 amino acid ~ 50 kDa enzymatic component of a multiprotein E3 ubiquitin ligase complex. Mutations in the PARK2 gene has been noted to affect susceptibility to Parkinson's disease (PARK) and development of Parkinson disease 2, also known as autosomal recessive juvenile Parkinsonism (PARK2) which differs from classical Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia (Kitada et al. 1998). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 288-388 of human PARK2
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Parkin antibody
-
Brody, K. M. et al (2008) Regional and cellular localisation of Parkin co-regulated gene in developing and adult mouse brain.
Brain Res. 1201: 177-86. -
Scuderi, S. et al. (2014) Alternative splicing generates different parkin protein isoforms: evidences in human, rat, and mouse brain.
Biomed Res Int. 2014:690796.
- RRID
- AB_2159924
- UniProt
- O60260
- Entrez Gene
- PARK2
View more products with PARKIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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