Parkin antibody | 1H4
Mouse anti Human Parkin antibody, clone 1H4 recognizes human E3 ubiquitin-protein ligase parkin, also known as Parkin or Parkinson juvenile disease protein 2. Parkin is a 465 amino acid ~ 50 kDa enzymatic component of a multiprotein E3 ubiquitin ligase complex.
Mutations in the PARK2 gene has been noted to affect susceptibility to Parkinson's disease (PARK) and development of Parkinson disease 2, also known as autosomal recessive juvenile Parkinsonism (PARK2) which differs from classical Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia (Kitada et al. 1998).
Mutations in the PARK2 gene has been noted to affect susceptibility to Parkinson's disease (PARK) and development of Parkinson disease 2, also known as autosomal recessive juvenile Parkinsonism (PARK2) which differs from classical Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia (Kitada et al. 1998).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 288-388 of human PARK2
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of Parkin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Parkin antibody
-
Brody, K. M. et al (2008) Regional and cellular localisation of Parkin co-regulated gene in developing and adult mouse brain.
Brain Res. 1201: 177-86. -
Scuderi, S. et al. (2014) Alternative splicing generates different parkin protein isoforms: evidences in human, rat, and mouse brain.
Biomed Res Int. 2014:690796.
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