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N-Cadherin antibody | 13A9

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Anti Human N-Cadherin Antibody, clone 13A9 thumbnail image 1 Anti Human N-Cadherin Antibody, clone 13A9 thumbnail image 2 Anti Human N-Cadherin Antibody, clone 13A9 thumbnail image 3 Anti Human N-Cadherin Antibody, clone 13A9 thumbnail image 4
Anti Human N-Cadherin Antibody, clone 13A9 gallery image 1
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human N-cadherin antibody, clone 13A9 (MCA5698) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power).
Anti Human N-Cadherin Antibody, clone 13A9 gallery image 2
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human N-cadherin antibody, clone 13A9 (MCA5698) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power).
Anti Human N-Cadherin Antibody, clone 13A9 gallery image 3
Normalized signal intensity of N-Cadherin from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti Human N-Cadherin Antibody, clone 13A9 gallery image 4
Western blot analysis of N-Cadherin CRISPR knockout HEK293 (N-Cadherin KO) and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti N-Cadherin antibody (MCA5698) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with (A) 40 second and (B) 30 second exposure.

Mouse anti Human N-Cadherin

Product Type
Monoclonal Antibody
Clone
13A9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5698
Datasheet
C IF IP P* WB
SDS
0.2 mg loader

Mouse anti Human N-cadherin antibody, clone 13A9 recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.

N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells.

Mouse anti Human N-cadherin antibody, clone 13A9 studies  have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).

Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P19022
Q9Z1Y3
Entrez Gene
CDH2
Cdh2
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0008013 beta-catenin binding
GO:0016342 catenin complex
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042692 muscle cell differentiation
GO:0045294 alpha-catenin binding
GO:0045295 gamma-catenin binding
GO:0051149 positive regulation of muscle cell differentiation
GO:0005916 fascia adherens
GO:0007416 synapse assembly
GO:0016339 calcium-dependent cell-cell adhesion
GO:0019901 protein kinase binding
GO:0031641 regulation of myelination
GO:0032403 protein complex binding
GO:0032880 regulation of protein localization
GO:0035023 regulation of Rho protein signal transduction
GO:0044456 synapse part
GO:0050770 regulation of axonogenesis
GO:0051291 protein heterooligomerization
Regulatory
For research purposes only

Applications of N-Cadherin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 1/100 1/1000
  1. 1 This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA5698 detects a band of approximately 135-140kDa in human HeLa cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for N-Cadherin antibody

  1. Wahl, J.K. 3rd et al. (2003) N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
    J Biol Chem. 278 (19): 17269-76.
  2. Knudsen, K.A. et al. (1995) Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
    J Cell Biol. 130 (1): 67-77.
  3. Machell, N.H. et al. (2000) Developmental expression and distribution of N- and E-cadherin in the rat ovary.
    Biol Reprod. 63 (3): 797-804.
  4. Han, A.C. et al. (1997) Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
    Hum Pathol. 28 (6): 641-5.
  5. Peralta Soler, A. et al. (1995) The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
    Hum Pathol. 26 (12): 1363-9.
  6. Van Aken, E.H. et al. (2002) Structure and function of the N-cadherin/catenin complex in retinoblastoma.
    Invest Ophthalmol Vis Sci. 43 (3): 595-602.
  7. Shintani, Y. et al. (2006) Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells.
    Mol Biol Cell. 17: 2963-75.
  8. Sacco, P.A. et al. (1995) Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
    J Biol Chem. 270: 20201-6.
  9. Tian, G. et al. (2009) Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton.
    J Biol Chem. 284: 18980-93.
  10. Theisen, C.S. et al. (2007) NHERF links the N-cadherin/catenin complex to the platelet-derived growth factor receptor to modulate the actin cytoskeleton and regulate cell motility.
    Mol Biol Cell. 18: 1220-32.
  11. Nieman, M.T. et al. (1999) N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression.
    J Cell Biol. 147 (3): 631-44.
  12. Islam, S. et al. (1996) Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
    J Cell Biol. 135 (6 Pt 1): 1643-54.
  13. Peralta Soler, A. et al. (1999) P-cadherin expression in breast carcinoma indicates poor survival.
    Cancer. 86: 1263-72.

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