N-Cadherin antibody | 13A9
N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells.
Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).
Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0008013 beta-catenin binding
- GO:0016342 catenin complex
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- GO:0042692 muscle cell differentiation
- GO:0045294 alpha-catenin binding
- GO:0045295 gamma-catenin binding
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0005916 fascia adherens
- GO:0007416 synapse assembly
- GO:0016339 calcium-dependent cell-cell adhesion
- GO:0019901 protein kinase binding
- GO:0031641 regulation of myelination
- GO:0032403 protein complex binding
- GO:0032880 regulation of protein localization
- GO:0035023 regulation of Rho protein signal transduction
- GO:0044456 synapse part
- GO:0050770 regulation of axonogenesis
- GO:0051291 protein heterooligomerization
- For research purposes only
Applications of N-Cadherin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1 This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- MCA5698 detects a band of approximately 135-140kDa in human HeLa cell lysates.
Secondary Antibodies Available
Product Specific References
References for N-Cadherin antibody
Wahl, J.K. 3rd et al. (2003) N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
J Biol Chem. 278 (19): 17269-76.
Knudsen, K.A. et al. (1995) Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
J Cell Biol. 130 (1): 67-77.
Machell, N.H. et al. (2000) Developmental expression and distribution of N- and E-cadherin in the rat ovary.
Biol Reprod. 63 (3): 797-804.
Han, A.C. et al. (1997) Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
Hum Pathol. 28 (6): 641-5.
Peralta Soler, A. et al. (1995) The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
Hum Pathol. 26 (12): 1363-9.
Van Aken, E.H. et al. (2002) Structure and function of the N-cadherin/catenin complex in retinoblastoma.
Invest Ophthalmol Vis Sci. 43 (3): 595-602.
Shintani, Y. et al. (2006) Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells.
Mol Biol Cell. 17: 2963-75.
Sacco, P.A. et al. (1995) Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
J Biol Chem. 270: 20201-6.
Tian, G. et al. (2009) Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton.
J Biol Chem. 284: 18980-93.
Theisen, C.S. et al. (2007) NHERF links the N-cadherin/catenin complex to the platelet-derived growth factor receptor to modulate the actin cytoskeleton and regulate cell motility.
Mol Biol Cell. 18: 1220-32.
Nieman, M.T. et al. (1999) N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression.
J Cell Biol. 147 (3): 631-44.
Islam, S. et al. (1996) Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
J Cell Biol. 135 (6 Pt 1): 1643-54.
Peralta Soler, A. et al. (1999) P-cadherin expression in breast carcinoma indicates poor survival.
Cancer. 86: 1263-72.
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