Mucin 5AC antibody | 1-13M1

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Mouse anti Human Mucin 5AC

Product Type
Monoclonal Antibody
Clone
1-13M1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1746 C E P* WB* 0.1 mg
Mouse anti Human Mucin 5AC antibody, clone 1-13M1 recognizes the peptide core of gastric mucin, also knowen as Mucin-5AC, Lewis B blood group antigen, Major airway glycoprotein or Tracheobronchial mucin.

Mucins are high molecular weight glycoproteins found predominantly in the secretions of mucus membranes, protecting them from infection or chemical damage. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. Mouse anti Human Mucin 5AC antibody, clone 1-13M1 has not been cross-adsorbed and may react with related peptides from other species.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Immunogen
A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P98088 Related reagents
Entrez Gene
MUC5AC Related reagents
Regulatory
For research purposes only

Applications of Mucin 5AC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 2
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
  2. 2Clone 1-13M1 recognizes Mucin 5AC under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Mucin 5AC antibody

  1. Fang, C. et al. (2012) Resistin-like molecule-β is a human airway remodelling mediator.
    Eur Respir J. 39 (2): 458-66.
  2. Ramaglia L et al. (2015) Differentiation, apoptosis, and GM-CSF receptor expression of human gingival fibroblasts on a titanium surface treated by a dual acid-etched procedure.
    Clin Oral Investig. Apr 17. [Epub ahead of print]