MDM2 antibody | SMP14

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Anti MDM2 Antibody, clone SMP14 gallery image 1 — Staining of human breast carcinoma. Formalin fixed, paraffin processed tissue with Mouse anti Human MDM2 (**MCA1709T**)

Mouse anti MDM2

Product Type: Monoclonal Antibody
Clone: SMP14
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1709T	C P* RE			20 µg
MCA1709	C IP P* RE			0.1 mg

Mouse anti MDM2 antibody, clone SMP14 recognizes the 90kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition.

MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI.

MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q00987 Related reagents
Entrez Gene: MDM2 Related reagents
Regulatory: For research purposes only

Applications of MDM2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin ¹	1/200	1/500
Immunohistology - Resin
Immunoprecipitation

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Breast Carcinoma

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for MDM2 antibody

Picksley, S.M. et al. (1994) Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.
Oncogene. 9 (9): 2523-9.
Martin, K. et al. (1995) Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein.
Nature. 375 (6533): 691-4.
Gueven, N. et al. (2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype.
Cancer Res. 66: 2907-12.

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