MCP-3 antibody | h.mcp.3

Mouse anti Human MCP-3

Product Type
Monoclonal Antibody
Clone
h.mcp.3
Isotype
IgG1
Specificity
MCP-3

Product Code Applications Pack Size List Price Your Price Qty
MCA1818
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human MCP-3 antibody, clone h.mcp.3 recognizes human C-C motif chemokine 7, also known as Monocyte chemoattractant protein 3, MCP-3, CCL7, NC28 or Small-inducible cytokine A7. MCP-3 is a 99 amino acid (including signal peptide sequence) ~9kDa chemotactic glycoprotein for monocytes and eosinophils. No cross reactivity is observed with MCP-1, MCP-2 or other structurally related chemokines.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human MCP-3 (Macrophage Chemoattractant Protein-3).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised C3H/HeJ mice were fused with cells of a mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Functional Assays 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for MCP-3 antibody

  1. Al-Bannawi, A. et al. (2011) Chlamydia pneumoniae induces chemokine expression by platelets in patients with atherosclerosis.
    Med Princ Pract. 20 (5): 438-43.

Further Reading

  1. Opdenakker, G. et al. (1993) Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines.
    Biochem Biophys Res Commun. 191: 535-42.

Synonyms
CCL7
RRID
AB_2259751
UniProt
P80098
Entrez Gene
CCL7
GO Terms
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0008201 heparin binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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