MCP-3 antibody | h.mcp.3
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Mouse anti Human MCP-3
- Product Type
- Monoclonal Antibody
- Clone
- h.mcp.3
- Isotype
- IgG1
- Specificity
- MCP-3
Antibody Quality and Performance Guarantee
| Mouse anti Human MCP-3 antibody, clone h.mcp.3 recognizes human C-C motif chemokine 7, also known as Monocyte chemoattractant protein 3, MCP-3, CCL7, NC28 or Small-inducible cytokine A7. MCP-3 is a 99 amino acid (including signal peptide sequence) ~9kDa chemotactic glycoprotein for monocytes and eosinophils. No cross reactivity is observed with MCP-1, MCP-2 or other structurally related chemokines. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human MCP-3 (Macrophage Chemoattractant Protein-3).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised C3H/HeJ mice were fused with cells of a mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/1000 | |
| Functional Assays | |
1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for MCP-3 antibody
-
Al-Bannawi, A. et al. (2011) Chlamydia pneumoniae induces chemokine expression by platelets in patients with atherosclerosis.
Med Princ Pract. 20 (5): 438-43.
Further Reading
-
Opdenakker, G. et al. (1993) Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines.
Biochem Biophys Res Commun. 191: 535-42.
- Synonyms
- CCL7
- RRID
- AB_2259751
- UniProt
- P80098
- Entrez Gene
- CCL7
- GO Terms
- GO:0007165 signal transduction
- GO:0005615 extracellular space
- GO:0006874 cellular calcium ion homeostasis
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
- GO:0008201 heparin binding
- View More GO Terms
MCA1818
160483
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with MCP-3 specificity
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