MAdCAM-1 antibody | 314G8
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Mouse anti Human MAdCAM-1:Biotin
Mouse anti Human MAdCAM-1:Low Endotoxin
Mouse anti Human MAdCAM-1
- Product Type
- Monoclonal Antibody
- Clone
- 314G8
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2320B | E | 0.1 mg |
|
|
| MCA2320EL | E FN | 0.5 mg |
|
|
| MCA2320 | C E | 0.2 mg |
|
|
| MCA2320T | C E F | 25 µg |
|
Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant soluble MAdCAM-1-fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13477
- Entrez Gene
- MADCAM1
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005624 membrane fraction
- GO:0006955 immune response
- GO:0050776 regulation of immune response
- Regulatory
- For research purposes only
Applications of MAdCAM-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 10ug/ml | |
| ELISA | |||
| Functional Assays | |||
| ELISA | |||
| Flow Cytometry | 1/10 | 1/100 | |
| Immunohistology - Frozen | 1/25 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for MAdCAM-1 antibody
-
Leung, E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts.
Immunol Cell Biol. 74 (6): 490-6. -
Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
Immunol Cell Biol. 82 (4): 400-9. -
Olloquequi, J. et al. (2011) Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients.
Eur Respir J. 37 (2): 289-98. -
Guerra-Pérez, N. et al. (2015) Retinoic Acid Imprints a Mucosal-like Phenotype on Dendritic Cells with an Increased Ability To Fuel HIV-1 Infection.
J Immunol. pii: 1402623. -
Goode, D. et al. (2014) Sex hormones selectively impact the endocervical mucosal microenvironment: implications for HIV transmission.
PLoS One. 9 (5): e97767. -
Guzzo, C. et al. (2017) Virion incorporation of integrin αα4β7 facilitates HIV-1 infection and intestinal homing.
Sci Immunol. 2 (11) [Epub ahead of print].
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