MAdCAM-1 antibody | 17F5

Mouse anti Human MAdCAM-1

Product Type
Monoclonal Antibody
Clone
17F5
Isotype
IgG1
Specificity
MAdCAM-1

Product Code Applications Pack Size List Price Your Price Qty
MCA2321
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SDS Safety Datasheet SDS
E F P WB * 0.2 mg loader
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loader
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Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a ~60 kDa transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes the mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1 (Leung, E. et al. 2004).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Camel
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant soluble MAdCAM-1-fc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/100
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1
  1. 1 Clone 17F5 recognizes human MAdCAM-1 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
This product does not require protein digestion prior to staining. This product does not require HIER prior to staining.
Western Blotting
MCA2321 detects a band of approximately 60kDa.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Mouse IgG1 Negative Control

References for MAdCAM-1 antibody

  1. Leung, E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts.
    Immunol Cell Biol. 74 (6): 490-6.
  2. Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
    Immunol Cell Biol. 82 (4): 400-9.
  3. Al-Mohammed Salem, K.T. et al. (2012) Adhesion Molecules and the Cellular Population of the Normal Camel (Camelus dromedaries) Mammary Glands
    The Open Veterinary Science Journal. 6 (1): 15-22.
  4. Guerra-Pérez N et al. (2015) Retinoic acid imprints a mucosal-like phenotype on dendritic cells with an increased ability to fuel HIV-1 infection.
    J Immunol. 194 (5): 2415-23.
  5. Loiseau, C. et al. (2016) CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected individuals.
    Mucosal Immunol. 9 (5): 1137-50.

Synonyms
Mucosal Addressin Cell Adhesion MOLECULE-1
RRID
AB_567121
UniProt
Q13477
Entrez Gene
MADCAM1
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005624 membrane fraction
GO:0006955 immune response
GO:0050776 regulation of immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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