Macrophages/Monocytes/Granulocytes antibody | MAC387

















Mouse anti Human Macrophages:Alexa Fluor® 488
Mouse anti Human Macrophages:APC
Mouse anti Human Macrophages:FITC
Mouse anti Human Macrophages:Low Endotoxin
Mouse anti Human Macrophages
Mouse anti Human Macrophages:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MAC387
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA874A488T | F* | 25 Tests/0.25ml |
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MCA874A488 | F* | 100 Tests/1ml |
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MCA874APC | F* | 100 Tests |
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MCA874FT | F* | 25 µg |
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MCA874F | F* | 0.1 mg |
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MCA874EL | C F* P* | 0.5 mg |
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MCA874GT | C F* P* | 20 µg |
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MCA874GA | C F* P* | 0.1 mg |
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MCA874G | C F* P* | 0.2 mg |
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MCA874PE | F* | 100 Tests |
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The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Horse Pig Dog Rabbit Baboon Bovine Guinea Pig Rat Cat Cynomolgus monkey Rhesus Monkey Goat Fallow deer Pygmy hippopotamus Mink Marmoset - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Reconstitution
- Reconstitute with 1.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Human monocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 20 µgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mg, 0.2 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06702
- Entrez Gene
- S100A9
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0005576 extracellular region
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005856 cytoskeleton
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0007267 cell-cell signaling
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Macrophages/Monocytes/Granulocytes antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | Neat | 1/5 | |
Flow Cytometry 1 | Neat | 1/5 | |
Flow Cytometry 1 | Neat | ||
Flow Cytometry 1 | 1/50 | 1/200 | |
Immunohistology - Frozen | 1/100 | 1/200 | |
Immunohistology - Paraffin 2 | 1/100 | 1/200 | |
Flow Cytometry 1 | 1/50 | 1/100 | |
Immunohistology - Frozen | 1/100 | 1/200 | |
Immunohistology - Paraffin 2 | 1/100 | 1/200 | |
Flow Cytometry 1 | Neat | 1/5 |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human Spleen
- Histology Positive Control Tissue
- Human Spleen
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
Flavell, D.J. et al. (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody.
J Histochem Cytochem. 35 (11): 1217-26.
Antibody Characterization Reference
-
Brandtzaeg, P. et al. (1988) Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen.
J Clin Pathol. 41 (9): 963-70.
References for Macrophages/Monocytes/Granulocytes antibody
-
Ueland, T. et al. (2009) Dickkopf-1 enhances inflammatory interaction between platelets and endothelial cells and shows increased expression in atherosclerosis.
Arterioscler Thromb Vasc Biol. 29: 1228-34 -
Brandtzaeg, P. et al. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function.
Histopathology. 21: 191-196. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Ramsay, A.D. et al. (1991) Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies.
J Pathol. 164 (4): 321-8. -
Christgau, M. et al. (1998) Characterization of immunocompetent cells in the diseased canine periodontium.
J Histochem Cytochem. 46 (12): 1443-54. -
Pérez, J. et al. (1999) Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma.
J Comp Pathol. 121 (4): 385-97. -
Nanney, L.B. et al. (2008) Calreticulin enhances porcine wound repair by diverse biological effects.
Am J Pathol. 173: 610-30. -
Poncelet, L. et al. (2008) Detection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
Vet Pathol. 45: 140-53. -
Sethi, R.S. et al. (2010) Immunolocalization of pulmonary intravascular macrophages, TLR4, TLR9 and IL-8 in normal and Pasteurella multocida-infected lungs of water buffalo (Bubalus bubalis).
J Comp Pathol. 144: 135-44. -
Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
J Comp Pathol. 145 (2-3): 107-17. -
Isling, L.K. et al. (2010) Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology.
Acta Vet Scand. 52: 48. -
Vranckx, K. et al. (2012) Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain.
BMC Vet Res. 8: 24. -
Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation.
J Clin Pathol. 65 (12): 1077-83. -
García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
Vet Immunol Immunopathol. 149: 66-75. -
Santana, C.H. et al. (2016) Relationship Between the Inflammatory Infiltrate and the Degree of Differentiation of the Canine Cutaneous Cell Carcinoma.
Vet Anim Sci. Oct 10 [Epub ahead of print] -
Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
PLoS Negl Trop Dis. 2013 Mar;7(3): e2138. -
Tellez, A. et al. (2014) Experimental evaluation of efficacy and healing response of everolimus-eluting stents in the familial hypercholesterolemic swine model: a comparative study of bioabsorbable versus durable polymer stent platforms.
Coron Artery Dis. 25 (3): 198-207. -
Collin, N. et al. (2009) Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania.
PLoS Pathog. 5(5):e1000441. -
McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
J Zoo Wildl Med. 45 (4): 906-10. -
Marcaccini, A. et al. (2008) Pseudorabies virus infection in mink: a host-specific pathogenesis.
Vet Immunol Immunopathol. 124 (3-4): 264-73. -
Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. Aug 25. [Epub ahead of print] -
Rossi, C.N. et al. (2016) In situ Cutaneous cellular immune response in dogs naturally infected by visceral leishmaniasis.
Rev Inst Med Trop Sao Paulo. 58: . -
Vrolyk V et al. (2016) Lung Inflammation Associated With Clinical Acute Necrotizing Pancreatitis in Dogs.
Vet Pathol. May 11. pii: 0300985816646432. [Epub ahead of print] -
Nelson, M. et al. (2014) Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus).
Int J Exp Pathol. 95 (6): 378-91. -
Amarilla, S.P. et al. (2016) Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains.
Vet Microbiol. 188: 47-58. -
García-Jiménez, W.L. et al. (2013) Immunopathology of granulomas produced by Mycobacterium bovis in naturally infected wild boar.
Vet Immunol Immunopathol. 156 (1-2): 54-63. -
Pilling, D. et al. (2015) The long pentraxin PTX3 promotes fibrocyte differentiation.
PLoS One. 10 (3): e0119709.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
Cytometry A. 83 (1): 103-13. -
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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