Macrophages/Monocytes/Granulocytes antibody | MAC387

Mouse anti Human Macrophages

Product Type
Monoclonal Antibody
Clone
MAC387
Isotype
IgG1
Specificity
Macrophages/Monocytes/Granulocytes

Product Code Applications Pack Size List Price Your Price Qty
MCA874GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P * 0.1 mg loader
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loader
MCA874G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P * 0.2 mg loader
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loader
MCA874GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P * 20 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.

The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Pig
Dog
Rabbit
Baboon
Bovine
Guinea Pig
Rat
Cat
Cynomolgus monkey
Rhesus Monkey
Goat
Fallow deer
Pygmy hippopotamus
Mink
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA874GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
MCA874G, MCA874GT: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human monocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen 1/100 1/200
Immunohistology - Paraffin 2 1/100 1/200
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human Spleen

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Description Goat anti Mouse IgG (Fc):FITC
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Source Reference

  1. Flavell, D.J. et al. (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody.
    J Histochem Cytochem. 35 (11): 1217-26.

Antibody Characterization Reference

  1. Brandtzaeg, P. et al. (1988) Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen.
    J Clin Pathol. 41 (9): 963-70. J Clin Pathol. 41 (9): 963-70.

References for Macrophages/Monocytes/Granulocytes antibody

  1. Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.
    J Virol. 76: 12233-41.
  2. Ueland, T. et al. (2009) Dickkopf-1 enhances inflammatory interaction between platelets and endothelial cells and shows increased expression in atherosclerosis.
    Arterioscler Thromb Vasc Biol. 29: 1228-34
  3. Brandtzaeg, P. et al. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function.
    Histopathology. 21: 191-6.
  4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
    Vet Immunol Immunopathol. 71 (3-4): 321-34.
  5. Ramsay, A.D. et al. (1991) Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies.
    J Pathol. 164 (4): 321-8.
  6. Christgau, M. et al. (1998) Characterization of immunocompetent cells in the diseased canine periodontium.
    J Histochem Cytochem. 46 (12): 1443-54.
  7. Pérez, J. et al. (1999) Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma.
    J Comp Pathol. 121 (4): 385-97.
  8. Nanney, L.B. et al. (2008) Calreticulin enhances porcine wound repair by diverse biological effects.
    Am J Pathol. 173: 610-30.
  9. View The Latest Product References
  10. Poncelet, L. et al. (2008) Detection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
    Vet Pathol. 45: 140-53.
  11. Sethi, R.S. et al. (2010) Immunolocalization of pulmonary intravascular macrophages, TLR4, TLR9 and IL-8 in normal and Pasteurella multocida-infected lungs of water buffalo (Bubalus bubalis).
    J Comp Pathol. 144: 135-44.
  12. Sanchez, J. et al. (2011) Microscopical and immunological features of tuberculoid granulomata and cavitary pulmonary tuberculosis in naturally infected goats.
    J Comp Pathol. 145 (2-3): 107-17.
  13. Isling, L.K. et al. (2010) Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology.
    Acta Vet Scand. 52: 48.
  14. Vranckx, K. et al. (2012) Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain.
    BMC Vet Res. 8: 24.
  15. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation.
    J Clin Pathol. 65 (12): 1077-83.
  16. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
    Vet Immunol Immunopathol. 149: 66-75.
  17. Santana, C.H. et al. (2016) Relationship Between the Inflammatory Infiltrate and the Degree of Differentiation of the Canine Cutaneous Cell Carcinoma.
    Vet Anim Sci. Oct 10 [Epub ahead of print]
  18. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
    PLoS Negl Trop Dis. 2013 Mar;7(3): e2138.
  19. Tellez, A. et al. (2014) Experimental evaluation of efficacy and healing response of everolimus-eluting stents in the familial hypercholesterolemic swine model: a comparative study of bioabsorbable versus durable polymer stent platforms.
    Coron Artery Dis. 25 (3): 198-207.
  20. Collin, N. et al. (2009) Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania.
    PLoS Pathog. 5(5):e1000441.
  21. McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
    J Zoo Wildl Med. 45 (4): 906-10.
  22. Marcaccini, A. et al. (2008) Pseudorabies virus infection in mink: a host-specific pathogenesis.
    Vet Immunol Immunopathol. 124 (3-4): 264-73.
  23. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  24. Rossi, C.N. et al. (2016) In situ Cutaneous cellular immune response in dogs naturally infected by visceral leishmaniasis.
    Rev Inst Med Trop Sao Paulo. 58: .
  25. Vrolyk V et al. (2016) Lung Inflammation Associated With Clinical Acute Necrotizing Pancreatitis in Dogs.
    Vet Pathol. May 11. pii: 0300985816646432. [Epub ahead of print]
  26. Nelson, M. et al. (2014) Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus).
    Int J Exp Pathol. 95 (6): 378-91.
  27. Amarilla, S.P. et al. (2016) Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains.
    Vet Microbiol. 188: 47-58.
  28. García-Jiménez, W.L. et al. (2013) Immunopathology of granulomas produced by Mycobacterium bovis in naturally infected wild boar.
    Vet Immunol Immunopathol. 156 (1-2): 54-63.
  29. Pilling, D. et al. (2015) The long pentraxin PTX3 promotes fibrocyte differentiation.
    PLoS One. 10 (3): e0119709.
  30. Zhao, L. et al. (2020) Reducing macrophage numbers alleviates temporomandibular joint ankylosis.
    Cell Tissue Res. 379 (3): 521-36.
  31. Lai, H.Y. et al. (2017) CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in M1 macrophages of vascular lesions.
    Cardiovasc Res. 113 (11): 1376-88.
  32. Wacinski, P. et al. (2021) Anti-Inflammatory Effect of Very High Dose Local Vessel Wall Statin Administration: Poly(L,L-Lactide) Biodegradable Microspheres with Simvastatin for Drug Delivery System (DDS).
    Int J Mol Sci. 22 (14)Jul 13 [Epub ahead of print].
  33. Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
    Biomaterials. 279: 121204.
  34. Bertolo, P.H.L. et al. (2022) Influence of serum progesterone levels on the inflammatory response of female dogs with visceral leishmaniosis.
    Vet Parasitol. 302: 109658.
  35. do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
    Cytometry A. 83 (1): 103-13.
  2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunohistology - Paraffin

Synonyms
Calprotectin
RRID
AB_321963
UniProt
P06702
Entrez Gene
S100A9
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0005576 extracellular region
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007267 cell-cell signaling
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