Integrin Alpha V Beta 5 antibody | P1F6

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Mouse anti Human Integrin Alpha V Beta 5

Product Type
Monoclonal Antibody
Clone
P1F6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5355-2657 C E F IF IP 0.1 mg
Mouse anti Human integrin alpha V beta 5 antibody, clone P1F6 recognizes the integrin alpha V beta 5 holomolecule. ITGaVb5 acts as a receptor for fibronectin and has also been co-opted as a cellular receptor for adenovirus type C (Lyle and McCormick 2010).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Human UCLA P3 cells (lung carcinoma).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P18084 Related reagents
P06756 Related reagents
Entrez Gene
ITGAV Related reagents
ITGB5 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0032369 negative regulation of lipid transport
GO:0044419 interspecies interaction between organisms
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0050748 negative regulation of lipoprotein metabolic process
GO:0050900 leukocyte migration
GO:0006936 muscle contraction
GO:0007275 multicellular organismal development
Regulatory
For research purposes only

Applications of Integrin Alpha V Beta 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
For use on human carcinomas, normal epithelium and keratinocytes using acetone or paraformaldehyde fixed tissue sections.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for Integrin Alpha V Beta 5 antibody

  1. Aplin, J. D. et al. (1996) Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium
    Mol Hum Reprod 2: 527 - 534
  2. Weinacker, A. et al. (1994) Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor
    J Biol Chem 269: 6940 - 6948
  3. Wayner, E. A. et al. (1991) Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface
    J Cell Biol 113: 919 - 929