Integrin Alpha 9 Beta 1 antibody | Y9A2
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Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 recognizes the human alpha9 integrin, a ~140 kDa glycoprotein expressed as a heterodimer in association with the beta1 integrin. The alpha9 integrin is expressed by epithelial cells, including airway epithelial cells.
Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 inhibits adhesion of alpha9 expressing cells to N-terminal osteopontin. Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 has been shown to cause 100% inhibition of α9β1-mediated adhesion to the third fibronectin type III repeat in tenascin at <10ug/ml (Yokosaki et al. 1998) and demonstrates Itgα9 blocking of progenitor cell proliferation (Schreiber et al. 2009).
Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 inhibits adhesion of alpha9 expressing cells to N-terminal osteopontin. Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 has been shown to cause 100% inhibition of α9β1-mediated adhesion to the third fibronectin type III repeat in tenascin at <10ug/ml (
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- alpha9 integrin transfected lambda cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13797
- Entrez Gene
- ITGA9
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- Regulatory
- For research purposes only
Applications of Integrin Alpha 9 Beta 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for Integrin Alpha 9 Beta 1 antibody
-
Wang, A. et al. (1996) Differential regulation of airway epithelial integrins by growth factors.
Am J Respir Cell Mol Biol. 15 (5): 664-72. -
Smith, L.L. et al. (1996) Osteopontin N-terminal domain contains a cryptic adhesive sequence recognized by alpha9beta1 integrin.
J Biol Chem. 271 (45): 28485-91. -
Yokosaki, Y. et al. (1996) Differential effects of the integrins alpha9beta1, alphavbeta3, and alphavbeta6 on cell proliferative responses to tenascin. Roles of the beta subunit extracellular and cytoplasmic domains.
J Biol Chem. 271 (39): 24144-50. -
Sime, W. et al. (2009) Human mast cells adhere to and migrate on epithelial and vascular basement membrane laminins LM-332 and LM-511 via alpha3beta1 integrin.
J Immunol. 183 (7): 4657-65.
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