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Integrin Alpha 9 Beta 1 antibody | Y9A2

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Mouse anti Human Integrin Alpha 9 Beta 1

Product Type
Monoclonal Antibody
Clone
Y9A2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1585T
Datasheet
F IP
MSDS
25 µg loader

MCA1585
Datasheet
F IP
MSDS
0.2 mg loader

Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 recognizes the human alpha9 integrin, a ~140 kDa glycoprotein expressed as a heterodimer in association with the beta1 integrin. The alpha9 integrin is expressed by epithelial cells, including airway epithelial cells.

Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 inhibits adhesion of alpha9 expressing cells to N-terminal osteopontin. Mouse anti Human Integrin alpha 9 beta 1 antibody, clone Y9A2 has been shown to cause 100% inhibition of α9β1-mediated adhesion to the third fibronectin type III repeat in tenascin at <10ug/ml (Yokosaki et al. 1998) and demonstrates Itgα9 blocking of progenitor cell proliferation (Schreiber et al. 2009).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
alpha9 integrin transfected lambda cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q13797
Entrez Gene
ITGA9
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
Regulatory
For research purposes only

Applications of Integrin Alpha 9 Beta 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for Integrin Alpha 9 Beta 1 antibody

  1. Wang, A. et al. (1996) Differential regulation of airway epithelial integrins by growth factors.
    Am J Respir Cell Mol Biol. 15 (5): 664-72.
  2. Smith, L.L. et al. (1996) Osteopontin N-terminal domain contains a cryptic adhesive sequence recognized by alpha9beta1 integrin.
    J Biol Chem. 271 (45): 28485-91.
  3. Yokosaki, Y. et al. (1996) Differential effects of the integrins alpha9beta1, alphavbeta3, and alphavbeta6 on cell proliferative responses to tenascin. Roles of the beta subunit extracellular and cytoplasmic domains.
    J Biol Chem. 271 (39): 24144-50.
  4. Sime, W. et al. (2009) Human mast cells adhere to and migrate on epithelial and vascular basement membrane laminins LM-332 and LM-511 via alpha3beta1 integrin.
    J Immunol. 183 (7): 4657-65.
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