Insulin antibody | E2E3

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Mouse anti Human Insulin

Product Type: Monoclonal Antibody
Clone: E2E3
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1911G	C E P			0.1 mg

Mouse anti Human Insulin antibody, clone E2E3 recognizes insulin, a major metabolic hormone produced by B cells of the pancreas.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine
Pig
Rabbit

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
0.1%	Bovine Serum Albumin

Immunogen

Insulin conjugated to BSA.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01308 Related reagents
Entrez Gene: INS Related reagents
GO Terms: GO:0000165 MAPKKK cascade; GO:0002674 negative regulation of acute inflammatory response; GO:0006953 acute-phase response; GO:0005158 insulin receptor binding; GO:0005159 insulin-like growth factor receptor binding; GO:0005179 hormone activity; GO:0005615 extracellular space; GO:0005788 endoplasmic reticulum lumen; GO:0006006 glucose metabolic process; GO:0006112 energy reserve metabolic process; GO:0006521 regulation of cellular amino acid metabolic process; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0007267 cell-cell signaling; GO:0050796 regulation of insulin secretion; GO:0008284 positive regulation of cell proliferation; GO:0008286 insulin receptor signaling pathway; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0045909 positive regulation of vasodilation; GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade; GO:0015758 glucose transport; GO:0046326 positive regulation of glucose import; GO:0046889 positive regulation of lipid biosynthetic process; GO:0022898 regulation of transmembrane transporter activity; GO:0030307 positive regulation of cell growth; GO:0030335 positive regulation of cell migration; GO:0031018 endocrine pancreas development; GO:0031904 endosome lumen; GO:0031954 positive regulation of protein autophosphorylation; GO:0032148 activation of protein kinase B activity; GO:0032583 regulation of gene-specific transcription; GO:0033861 negative regulation of NAD(P)H oxidase activity; GO:0042060 wound healing; GO:0042177 negative regulation of protein catabolic process; GO:0042593 glucose homeostasis; GO:0043066 negative regulation of apoptosis; GO:0043410 positive regulation of MAPKKK cascade; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045597 positive regulation of cell differentiation; GO:0045721 negative regulation of gluconeogenesis; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0045740 positive regulation of DNA replication; GO:0045818 negative regulation of glycogen catabolic process; GO:0045821 positive regulation of glycolysis; GO:0045840 positive regulation of mitosis; GO:0045861 negative regulation of proteolysis; GO:0045908 negative regulation of vasodilation; GO:0045922 negative regulation of fatty acid metabolic process; GO:0046628 positive regulation of insulin receptor signaling pathway; GO:0046631 alpha-beta T cell activation; GO:0050709 negative regulation of protein secretion; GO:0050715 positive regulation of cytokine secretion; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050995 negative regulation of lipid catabolic process; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051897 positive regulation of protein kinase B signaling cascade; GO:0055089 fatty acid homeostasis; GO:0060266 negative regulation of respiratory burst involved in inflammatory response; GO:0060267 positive regulation of respiratory burst
Regulatory: For research purposes only

Applications of Insulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		(as detecting reagent 5ng/ml)
Immunohistology - Frozen	1/50	1/100
Immunohistology - Paraffin	1/50	1/100

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue: Pancreas

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Insulin antibody

McCluskey, J.T. et al. (2011) Development and functional characterization of insulin-releasing human pancreatic beta cell lines produced by electrofusion.
J Biol Chem. 286: 21982-92.

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