Insulin antibody | E2E3
Mouse anti Human Insulin antibody, clone E2E3 (MCA1911G) used to stain rat pancreatic islet grafts by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Islet graft histology at 28 days after transplantation.
From: Nishino T, Goi T, Morikawa M, Koneri K, Terada S, Murakami M.
Fructan Improves Survival and Function of Cryopreserved Rat Islets.
Nutrients. 2021 Aug 25;13(9):2959.
doi: 10.3390/nu13092959.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Insulin
- Product Type
- Monoclonal Antibody
- Clone
- E2E3
- Isotype
- IgG1
- Specificity
- Insulin
Antibody Quality and Performance Guarantee
| Mouse anti Human Insulin antibody, clone E2E3 recognizes insulin, a major metabolic hormone produced by B cells of the pancreas. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Pig Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
0.1% bovine serum albumin - Immunogen
- Insulin conjugated to BSA.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
(as detecting reagent 5ng/ml) | |
| Immunohistology - Frozen | |
1/50 | 1/100 |
| Immunohistology - Paraffin | |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human pancreas
References for Insulin antibody
-
Marigo, V. et al. (2009) TRPM4 impacts on Ca2+ signals during agonist-induced insulin secretion in pancreatic beta-cells.
Mol Cell Endocrinol. 299 (2): 194-203. -
McCluskey, J.T. et al. (2011) Development and functional characterization of insulin-releasing human pancreatic beta cell lines produced by electrofusion.
J Biol Chem. 286: 21982-92. -
Çakir, B. et al. (2012) Structure based discovery of small molecules to regulate the activity of human insulin degrading enzyme.
PLoS One. 7 (2): e31787. -
Ismail. H.I. & Alli, A.M. (2015) Immunohistochemical Studies on The Endocrine Cells in The Thymus of The One-Humped-Camel (Camelus dromedarius)
J Camel Pract Res. 22 (2): 251. -
Nishino, T. et al. (2021) Fructan Improves Survival and Function of Cryopreserved Rat Islets.
Nutrients. 13 (9): 2959.
- RRID
- AB_323672
- UniProt
- P01308
- Entrez Gene
- INS
- GO Terms
- GO:0000165 MAPKKK cascade
- GO:0002674 negative regulation of acute inflammatory response
- GO:0006953 acute-phase response
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0005788 endoplasmic reticulum lumen
- GO:0006006 glucose metabolic process
- View More GO Terms
- GO:0006112 energy reserve metabolic process
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007267 cell-cell signaling
- GO:0050796 regulation of insulin secretion
- GO:0008284 positive regulation of cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0045909 positive regulation of vasodilation
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- GO:0015758 glucose transport
- GO:0046326 positive regulation of glucose import
- GO:0046889 positive regulation of lipid biosynthetic process
- GO:0022898 regulation of transmembrane transporter activity
- GO:0030307 positive regulation of cell growth
- GO:0030335 positive regulation of cell migration
- GO:0031018 endocrine pancreas development
- GO:0031904 endosome lumen
- GO:0031954 positive regulation of protein autophosphorylation
- GO:0032148 activation of protein kinase B activity
- GO:0032583 regulation of gene-specific transcription
- GO:0033861 negative regulation of NAD(P)H oxidase activity
- GO:0042060 wound healing
- GO:0042177 negative regulation of protein catabolic process
- GO:0042593 glucose homeostasis
- GO:0043066 negative regulation of apoptosis
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045597 positive regulation of cell differentiation
- GO:0045721 negative regulation of gluconeogenesis
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0045740 positive regulation of DNA replication
- GO:0045818 negative regulation of glycogen catabolic process
- GO:0045821 positive regulation of glycolysis
- GO:0045840 positive regulation of mitosis
- GO:0045861 negative regulation of proteolysis
- GO:0045908 negative regulation of vasodilation
- GO:0045922 negative regulation of fatty acid metabolic process
- GO:0046628 positive regulation of insulin receptor signaling pathway
- GO:0046631 alpha-beta T cell activation
- GO:0050709 negative regulation of protein secretion
- GO:0050715 positive regulation of cytokine secretion
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050995 negative regulation of lipid catabolic process
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051897 positive regulation of protein kinase B signaling cascade
- GO:0055089 fatty acid homeostasis
- GO:0060266 negative regulation of respiratory burst involved in inflammatory response
- GO:0060267 positive regulation of respiratory burst
MCA1911G
149633
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