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Insulin antibody | E2E3

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Anti Human Insulin Antibody, clone E2E3 gallery image 1
Published customer image:
Mouse anti Human Insulin antibody, clone E2E3 (MCA1911G) used to stain rat pancreatic islet grafts by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Islet graft histology at 28 days after transplantation.

From: Nishino T, Goi T, Morikawa M, Koneri K, Terada S, Murakami M.
Fructan Improves Survival and Function of Cryopreserved Rat Islets.
Nutrients. 2021 Aug 25;13(9):2959.
doi: 10.3390/nu13092959.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human Insulin

Product Type
Monoclonal Antibody
Clone
E2E3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1911G
Datasheet
C E P
SDS
0.1 mg loader

Mouse anti Human Insulin antibody, clone E2E3 recognizes insulin, a major metabolic hormone produced by B cells of the pancreas.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Pig
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Insulin conjugated to BSA.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P01308
Entrez Gene
INS
GO Terms
GO:0000165 MAPKKK cascade
GO:0002674 negative regulation of acute inflammatory response
GO:0006953 acute-phase response
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0005788 endoplasmic reticulum lumen
GO:0006006 glucose metabolic process
GO:0006112 energy reserve metabolic process
GO:0006521 regulation of cellular amino acid metabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0050796 regulation of insulin secretion
GO:0008284 positive regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0045909 positive regulation of vasodilation
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0015758 glucose transport
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0022898 regulation of transmembrane transporter activity
GO:0030307 positive regulation of cell growth
GO:0030335 positive regulation of cell migration
GO:0031018 endocrine pancreas development
GO:0031904 endosome lumen
GO:0031954 positive regulation of protein autophosphorylation
GO:0032148 activation of protein kinase B activity
GO:0032583 regulation of gene-specific transcription
GO:0033861 negative regulation of NAD(P)H oxidase activity
GO:0042060 wound healing
GO:0042177 negative regulation of protein catabolic process
GO:0042593 glucose homeostasis
GO:0043066 negative regulation of apoptosis
GO:0043410 positive regulation of MAPKKK cascade
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045597 positive regulation of cell differentiation
GO:0045721 negative regulation of gluconeogenesis
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045818 negative regulation of glycogen catabolic process
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0045861 negative regulation of proteolysis
GO:0045908 negative regulation of vasodilation
GO:0045922 negative regulation of fatty acid metabolic process
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0046631 alpha-beta T cell activation
GO:0050709 negative regulation of protein secretion
GO:0050715 positive regulation of cytokine secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050995 negative regulation of lipid catabolic process
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0055089 fatty acid homeostasis
GO:0060266 negative regulation of respiratory burst involved in inflammatory response
GO:0060267 positive regulation of respiratory burst
Regulatory
For research purposes only

Applications of Insulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA (as detecting reagent 5ng/ml)
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Pancreas
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Insulin antibody

  1. McCluskey, J.T. et al. (2011) Development and functional characterization of insulin-releasing human pancreatic beta cell lines produced by electrofusion.
    J Biol Chem. 286: 21982-92.
  2. Nishino, T. et al. (2021) Fructan Improves Survival and Function of Cryopreserved Rat Islets.
    Nutrients. 13 (9): 2959.

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