Insulin antibody | E2E3

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Mouse anti Human Insulin

Product Type
Monoclonal Antibody
Clone
E2E3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1911G C E P 0.1 mg
Mouse anti Human Insulin antibody, clone E2E3 recognizes insulin, a major metabolic hormone produced by B cells of the pancreas.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Pig
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%Bovine Serum Albumin
Immunogen
Insulin conjugated to BSA.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01308 Related reagents
Entrez Gene
INS Related reagents
GO Terms
GO:0000165 MAPKKK cascade
GO:0002674 negative regulation of acute inflammatory response
GO:0006953 acute-phase response
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0005788 endoplasmic reticulum lumen
GO:0006006 glucose metabolic process
GO:0006112 energy reserve metabolic process
GO:0006521 regulation of cellular amino acid metabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0050796 regulation of insulin secretion
GO:0008284 positive regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0045909 positive regulation of vasodilation
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0015758 glucose transport
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0022898 regulation of transmembrane transporter activity
GO:0030307 positive regulation of cell growth
GO:0030335 positive regulation of cell migration
GO:0031018 endocrine pancreas development
GO:0031904 endosome lumen
GO:0031954 positive regulation of protein autophosphorylation
GO:0032148 activation of protein kinase B activity
GO:0032583 regulation of gene-specific transcription
GO:0033861 negative regulation of NAD(P)H oxidase activity
GO:0042060 wound healing
GO:0042177 negative regulation of protein catabolic process
GO:0042593 glucose homeostasis
GO:0043066 negative regulation of apoptosis
GO:0043410 positive regulation of MAPKKK cascade
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045597 positive regulation of cell differentiation
GO:0045721 negative regulation of gluconeogenesis
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045818 negative regulation of glycogen catabolic process
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0045861 negative regulation of proteolysis
GO:0045908 negative regulation of vasodilation
GO:0045922 negative regulation of fatty acid metabolic process
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0046631 alpha-beta T cell activation
GO:0050709 negative regulation of protein secretion
GO:0050715 positive regulation of cytokine secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050995 negative regulation of lipid catabolic process
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0055089 fatty acid homeostasis
GO:0060266 negative regulation of respiratory burst involved in inflammatory response
GO:0060267 positive regulation of respiratory burst
Regulatory
For research purposes only

Applications of Insulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA (as detecting reagent 5ng/ml)
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Pancreas

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Insulin antibody

  1. McCluskey, J.T. et al. (2011) Development and functional characterization of insulin-releasing human pancreatic beta cell lines produced by electrofusion.
    J Biol Chem. 286: 21982-92.