Mouse anti Human Insulin antibody, clone 7F8 (E6E5) recognizes insulin, a major metabolic hormone produced by B cells of the pancreas.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Human insulin.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
negative regulation of acute inflammatory response
insulin receptor binding
insulin-like growth factor receptor binding
endoplasmic reticulum lumen
glucose metabolic process
energy reserve metabolic process
regulation of cellular amino acid metabolic process
G-protein coupled receptor protein signaling pathway
regulation of insulin secretion
positive regulation of cell proliferation
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
positive regulation of vasodilation
positive regulation of phosphatidylinositol 3-kinase cascade
positive regulation of glucose import
positive regulation of lipid biosynthetic process
regulation of transmembrane transporter activity
positive regulation of cell growth
positive regulation of cell migration
endocrine pancreas development
positive regulation of protein autophosphorylation
activation of protein kinase B activity
regulation of gene-specific transcription
negative regulation of NAD(P)H oxidase activity
negative regulation of protein catabolic process
negative regulation of apoptosis
positive regulation of MAPKKK cascade
positive regulation of nitric oxide biosynthetic process
positive regulation of cell differentiation
negative regulation of gluconeogenesis
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
negative regulation of glycogen catabolic process
positive regulation of glycolysis
positive regulation of mitosis
negative regulation of proteolysis
negative regulation of vasodilation
negative regulation of fatty acid metabolic process
positive regulation of insulin receptor signaling pathway
alpha-beta T cell activation
negative regulation of protein secretion
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of lipid catabolic process
positive regulation of nitric-oxide synthase activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling cascade
fatty acid homeostasis
negative regulation of respiratory burst involved in inflammatory response
positive regulation of respiratory burst
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Insulin antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Insulin antibody
Oran, P.E. et al. (2011) Mass spectrometric immunoassay of intact insulin and related variants for population proteomics studies.
Proteomics Clin Appl. 5: 454-9.
Sienkiewicz, W.et al. (2000) Has active immunization against gonadotrophin-releasing hormone any effect on testis innervation in the pig? An immunohistochemical study.
Anat Histol Embryol. 29 (4): 247-54.
Pillon, N.J. et al. (2015) Palmitate-induced inflammatory pathways in human adipose microvascular endothelial cells promote monocyte adhesion and impair insulin transcytosis.
Am J Physiol Endocrinol Metab. 309 (1): E35-44.