IL-18BP antibody | B01-3E12

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Mouse anti Human INTERLEUKIN-18BP

Product Type
Monoclonal Antibody
Clone
B01-3E12
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6244GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-18BP antibody, clone B01-3E12, recognizes interleukin-18-binidng protein (IL-18BP), also known as tadekinig-alpha. IL-18BP is a secreted immunoregulatory binding protein which binds to and inhibits the cytokine activity of mature IL-18 with high affinity. IL-18 plays a role in host defense, inflammation, tissue repair, and regeneration (Mühl & Bachmann 2019).

IL-18BP is constitutively expressed in the spleen, PBMCs, monocytes and umbilical vein endothelial cells (Mühl & Bachmann 2019, Corbaz et al. 2002). It is detectable in the serum of healthy humans at a 20 fold molar excess compared to IL-18, with subjects showing levels of 2,000 – 4,000 pg/ml of IL-18BP (Kaplanski et al. 2018). IL-18BP is upregulated in response to inflammation as part of the inflammation resolution process. Additionally, it can be upregulated during various cancers and IL-18BP levels are also affected by viral infections such as dengue and hepatitis C (Mühl & Bachmann 2019).

The purified Mouse anti Human interleukin-18BP antibody, clone B01-3E12 (MCA6244GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-18BP antibody, clone A07-5D2 (MCA6245B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
NS0 derived recombinant human full length interleukin-18BP (Thr31-Gly194)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O95998
Entrez Gene
IL18BP
GO Terms
GO:0005576 extracellular region
GO:0042007 interleukin-18 binding
GO:0042088 T-helper 1 type immune response
GO:0048019 receptor antagonist activity
Regulatory
For research purposes only

Applications of IL-18BP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human INTERLEUKIN-18BP:Biotin MCA6245B E 0.1 mg loader

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