IL-17A/F antibody | D01-1C2

100% Secure


Mouse anti Human Interleukin-17A/F

Product Type
Monoclonal Antibody
Clone
D01-1C2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6206GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-17A/F antibody, clone D01-1C2, recognizes interleukin-17 A and F. The IL-17 family consists of six proteins (IL-17A to IL-17F) which function as proinflammatory cytokines, important for defence against pathogens (Gurczynski and Moore, 2017). Accordingly, IL-17-producing CD4+ T helper cells, also called Th17 cells, play important roles in immune defense. Uncontrolled IL-17 signaling is associated with immunopathology, autoimmune diseases, and cancer, and blocking activity of IL-17 can be beneficial in some clinical settings (Amatya et al. 2017). This antibody recognizes IL-17A, the most studied member of the IL-17 family, and its closest relative IL-17F (Chen and Kolls 2017). These two proteins have similar structure and inflammatory function, but unique functions of IL-17F in the immune system have also been indicated (Chang and Dong, 2009).

The purified Mouse anti Human interleukin-17A/F antibody, clone D01-1C2 (MCA6206GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-17A/F antibody, clone I07-5E4 (MCA6207B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length interleukin-17A/F (Gly24-Ala155)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q16552
Q96PD4
Entrez Gene
IL17A
IL17F
GO Terms
GO:0001775 cell activation
GO:0006915 apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006486 protein glycosylation
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0032747 positive regulation of interleukin-23 production
GO:0045672 positive regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0006029 proteoglycan metabolic process
GO:0016525 negative regulation of angiogenesis
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0019955 cytokine binding
GO:0042109 lymphotoxin A biosynthetic process
GO:0042803 protein homodimerization activity
GO:0045076 regulation of interleukin-2 biosynthetic process
GO:0045408 regulation of interleukin-6 biosynthetic process
GO:0045414 regulation of interleukin-8 biosynthetic process
GO:0045423 regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
GO:0051216 cartilage development
Regulatory
For research purposes only

Applications of IL-17A/F antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-17A/F:Biotin MCA6207B E 0.1 mg loader

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸


How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra